Sequences from confirmation amplicon


Confirmation sequences for line SALK_047972c and hit At2g32280 are shown.
Line ID SALK_047972c
Locus At2g32280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13713831
Primers used for PCR 16og - LBb3, show primer details
   
Sequence Name 47-K114681-SALK_047972C-16og
GenBank Accession LN485438 [GenBank]
Sequencing primer gene specific primer, 16og: 5'-TTAATTTAAAGACCCGAAGCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K114681-SALK_047972C-16og
ATTTTACCCCACATGCGACAACAGAGCGTGACATAAAAGCTTTTCCATATTTTTCAAAAT
GACACAGCTTTCAAAATTACAAAAGTGACTTCGAAATAAGTGAAACGATTCATAAGAAGA
AATAAATTGAAACTAGGTTATATGATTATACATAAATATAGTCAAGAACAAGGACCTACC
TGTTCCATCATTAACCGTGACACTTTGTCACTTAGCTTCATCTTTGGCCGCAGTGGCAGA
AACGTAATAAGCAACACAGAAGAGGGCATGAAGAAAACACAAAATCCCTCCAATGGAGAG
AAAATGGTGATGTGTGAATCCACAAGAGGATCTTGACTTACTGTTCGACATCGTAAACAA
ATCATGTCAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCG
   
Sequence Name 23-K114680-SALK_047972C-R210
GenBank Accession LN484951 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114680-SALK_047972C-R210
GATTTGTTACGATGTCGAACAGTAAGTCAAGATCCTCTTGTGGATTCACACATCACCATT
TTCTCTCCATTGGAGGGATTTTGTGTTTTCTTCATGCCCTCTTCTGTGTTGCTTATTACG
TTTCTGCCACTGCGGCCAAAGATGAAGCTAAGTGACAAAGTGTCACGGTTAATGATGGAA
CAGGTAGGTCCTTGTTCTTGACTATATTTATGTATAATCATATAACCTAGTTTCAATTTA
TTTCTTCTTATGAATCGTTTCACTTATTTCGAAGTCACTTTTGTAATTTTGAAAGCTGTG
TCATTTTGAAAAATATGGAAAAGCTTTTATGTCACGCTCTGTTGTCGCATGTGGGGTTAA
AATGCACTAGTGATAATGACATTCGGTGGAATCTTGTCTTTGCTTCGGGTCTTTAAATTA

Last Updated on 06.12.2018 16:11