Sequences from confirmation amplicon


Confirmation sequences for line SALK_048387c and hit At1g62960 are shown.
Line ID SALK_048387c
Locus At1g62960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23320473
Primers used for PCR 24ea - LBb3, show primer details
   
Sequence Name 63-K110715-SALK_048387C-24ea
GenBank Accession LN485818 [GenBank]
Sequencing primer gene specific primer, 24ea: 5'-GATTCTCTAAAACCCAATCATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K110715-SALK_048387C-24ea
GAGAAGAATCAGTCAATCCGGAAATAATCACACAAAAAGTTTCAATTTTTTTTGAAGAAA
TTTAGATACAAAACCTTGTTGTTTTGAGCTAAACCAAGTTGGATAACTCCGTCCGGGTTA
CCCAACTCATCATACGGATCATCCTCGACCCGTTTTTGACCAACGTAGTAAGGCGAAGAA
TCTCCACCTTTGAGTACACAATTAACCCGACCCGAAACCGAAACAGCTCCACCGGAATTT
CTCGGCGAGAGGAGAATACGAGAGAGAGAACGAGGTAAACCAGTAAGCTCCGTGAGATGA
CCAGCGGATTGTGACCGGGTGGGATCCGGTAAACCCGAATTAGGAGAAGAAGAAGAAGAA
GCTGAGGAGTTTTGTTCACCGGAATTATCTGATTCGTCGTTTTTACGATTTCGTTTTAGG
TAAAACTGAAGAAAGTAGAAGAAAGCACAAGGAATCACAGAGCCTAAGAATAAACCACCA
CGACCTTGTACCACACCTTGTAACGGAACAATAACCCTCATCCCGGTGGTTCTACCTCCG
CCGACGTTACCGGAATTCTTATCGGAATCGGAATTGGAGCTCCGGCTCCGGTTTGGTTCG
GTACGGGTCATTGTAGTGAACTCATGTTTTTAGGTAAATAAATATTTTAGTGTTTGAAAT
CAAAATCGGAGCCACTTTGAAGGTTTTTTAACGGGTCGGGTTAACGGATAATTGAGTCCG
ACGGCGAAGAGATTTGAAAGAGAAAGAGAAGAGTTTTGATTTTTAGGTTAAAAGAAAATA
AAATTCGAATTTGTTTGTTTTTGCTTTTTGGATTTTTGTTTAATTTCTGATCTGTTTTAT
TTCGTACGCTCGTTTGTGGTGTAAACAAATTGACGCTNAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTA
   
Sequence Name 47-K110714-SALK_048387C-R210
GenBank Accession LN485434 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K110714-SALK_048387C-R210
GTCATTTGTTTACCCCAAACGAGCGTACGAAATAAAACAGATCAGAAATTAAACAAAAAT
CCAAAAAGCAAAAACAAACAAATTCGAATTTTATTTTCTTTTAACCTAAAAATCAAAACT
CTTCTCTTTCTCTTTCAAATCTCTTCGCCGTCGGACTCAATTATCCGTTAACCCGACCCG
TTAAAAAACCTTCAAAGTGGCTCCGATTTTGATTTCAAACACTAAAATATTTATTTACCT
AAAAACATGAGTTCACTACAATGACCCGTACCGAACCAAACCGGAGCCGGAGCTCCAATT
CCGATTCCGATAAGAATTCCGGTAACGTCGGCGGAGGTAGAACCACCGGGATGAGGGTTA
TTGTTCCGTTACAAGGTGTGGTACAAGGTCGTGGTGGTTTATTCTTAGGCTCTGTGATTC
CTTGTGCTTTCTTCTACTTTCTTCAGTTTTACCTAAAACGAAATCGTAAAAACGACGAAT
CAGATAATTCCGGTGAACAAAACTCCTCAGCTTCTTCTTCTTCTTCTCCTAATTCGGGTT
TACCGGATCCCACCCGGTCACAATCCGCTGGTCATCTCACGGAGCTTACTGGTTTACCTC
GTTCTCTCTCTCGTATTCTCCTCTCGCCGAGAAATTCCGGTGGAGCTGTTTCGGTTTCGG
GTCGGGTTAATTGTGTACTCAAAGGTGGAGATTCTTCGCCTTACTACGTTGGTCAAAAAC
GGGTCGAGGATGATCCGTATGATGAGTTGGGTAACCCGGACGGAGTTATCCAACTTGGTT
TAGCTCANAACAACAAGGTTTTGTATCTAAATTTCTTCAAAAAAAATTGAAACTTTTTGT
GTGATTATTTCCTGGATTTGACTGATCTCTTTCTTCTTATAATAGTTGAGTTTGGATGAT
T

Last Updated on 06.12.2018 16:11