Sequences from confirmation amplicon


Confirmation sequences for line SALK_050243c and hit At4g00390 are shown.
Line ID SALK_050243c
Locus At4g00390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:171511
Primers used for PCR 29ux - LBb3, show primer details
   
Sequence Name 16-K117668-SALK_050243C-R210
GenBank Accession LN484727 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K117668-SALK_050243C-R210
CACAATATATCCTGAAACAAATATTATAACGTAGAGAATCTTTCAACCCCAAAAAAACAA
ACCAGAAAGAATCAAATCCTAAATCCGAAAAACAAAAGAAAGCATTATGCATAGCATTAC
GATACAAAAAACCGACAAATCTAAGTAACTAGTAGCTCTAGTTCTTCGAAGCATCAACGA
AGTAAGACGTAAGGTCAGTCAAACGCACACTCTTATCCAACATAGCCTCAAGCTCTTTAG
CTTGCATCAACTGAAACTTCTCTTCAACAATCTTCTTAGTCTCAAGAGTAAACGAACTCC
ATTTCTGCCTCACATAATACTCATCAATACCCGAACCAGCAATCATCCTAGCAAGAGACG
ACTTCTCAAACCAATCATCTCCATTAGTTGAAGAAACACCAACAAACGCCAAGTCTTGCT
TTACAGAATCAAGCTTCTCCTTCGTCTTACTCGCACTCTTTTTCGACACACCGTTGGACT
TAGCATTAGAATCAAGAGCTATTCCTTTAGGTCCCCAAATAAACTTTGACAATTCAAAAG
CTTTCTTATCATGAGCTTTCACAAACCTAGGTTCGTCCTTTCCTTCTTTAGCACGGTACT
TCTTCCTTAAACTCCTAACCTTATCCGAGAATTGACTCTTACTAACCTCAAAGCTGATAG
ATTTCTTCTGGAACTCGTAAAACGCATTAGTATCTGCGGAAGGAGACTTCCCTGTATCAG
CATGATAATCTATCATTCCTTGTAACATAGAGATTTCGTCATCGTCACTC
   
Sequence Name 53-K117669-SALK_050243C-29ux
GenBank Accession LN485588 [GenBank]
Sequencing primer gene specific primer, 29ux: 5'-TTCAAAGACTATGGAGTGACGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K117669-SALK_050243C-29ux
ATTTCATGCTGATACAGGGAAGTCTCCTTCCGCAGATACTAATGCGTTTTACGAGTTCCA
GAAGAAATCTATCAGCTTTGAGGTTAGTAAGAGTCAATTCTCGGATAAGGTTAGGAGTTT
AAGGAAGAAGTACCGTGCTAAAGAAGGAAAGGACGAACCTAGGTTTGTGAAAGCTCATGA
TAAGAAAGCTTTTGAATTGTCAAAGTTTATTTGGGGACCTAAAGGAATAGCTCTTGATTC
TAATGCTAAGTCCAACGGTGTGTCGAAAAAGAGTGCGAGTAAGACGAAGGAGAAGCTTGA
TTCTGTAAAGCAAGACTTGGCGTTTGTTGGTGTTTCTTCAACTAATGGAGATGATTGGTT
TGAGAAGTCGTCTCTTGCTAGGATGATTGCTGGTTCGGGTATTGATGAGTATTATGTGAG
GCAGAAATGGAGTTCGTTTACTCTTGAGACTAAGAAGATTGTTGAAGAGAAGTTTCAGTT
GATGCAAGCTAAAGAGCTTGAGGCTATGTTGGATAAGAGTGTGCGTTTGACTGACCTTAC
GTCTTACTTCGTTGATGCTTCGAAGAACTAGAGCTACTAGTTACTTAGATTTGTCGGTTT
TTTGTATCGTAATGCTATGCATAATGCTTTCTTTTGTTTTTCGGATTTAGGATTTGATTC
TTTCTGGTTTGTTTTTTTGGGGTTGAAAGATTCTCTACGTTATAATATTTGTTTCAGGAT
ATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
ATGTACTGGGGTGGTTTT

Last Updated on 06.12.2018 16:11