Sequences from confirmation amplicon


Confirmation sequences for line SALK_054396 and hit At1g73570 are shown.
Line ID SALK_054396
Locus At1g73570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27653828
Primers used for PCR 14jg - LBb3, show primer details
   
Sequence Name 06-K113376-SALK_054396C-14jg
GenBank Accession LN484433 [GenBank]
Sequencing primer gene specific primer, 14jg: 5'-TATGCTAAATCTCAATCAAATGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113376-SALK_054396C-14jg
GGAGAGGTCTTCCTTTTGATCTCCATCTTGCTAAACGTTACTACGACCAGGCCCTCCAGA
GCGATACTGCTGCCAAATTGCCCGTCACGCTTGCTCTTGCGAGTGTATGGGTCCGCAGAA
ACTATGCTGATACTGCCCTGGTGAGAGAGAGGGAAGGAGAGTGATGATTTATCTGTTAAT
GGAATTGTCCGAGTGAAAATGGATGACCAAGGTCACGTTTTATGGATGCAGGTGCAAGTA
CTGAATTCCTTACCAGAAGTGCATCAAAAGGTGGTGGAATGGGTAGAGAATGGGATGTTG
GAAGAAGGTAATGCGACCATACTAACTCTTTTCCTTTGTAAACAAATTGACGCTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAA
AA
   
Sequence Name 02-K113375-SALK_054396C-R210
GenBank Accession LN484310 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113375-SALK_054396C-R210
CATTTGTTACAAGGAAAGAGTTAGTATGGTCGCATTACCTTCTTCCACATCCCATTCTCT
ACCCATTCCACCACCTTTTGATGCACTTCTGGTAAGGAATTCAGTACTTGCACCTGCATC
CATAAAACGTGACCTTGGTCATCCATTTTCACTCGGACAATTCCATTAACAGATAAATCA
TCACTCTCCTTCCCTCTCTCTCACCAGGGCAGTATCAGCATAGTTTCTGCGGACCCATAC
ACTCGCAAGAGCAAGCGTGACGGGCAATTTGGCAGCAGTATCGCTCTGGAGGGCCTGGTC
GTAGTAACGTTTAGCAAGATGGAGATCAAAAGGAAGACCTTCTCCATGCTCATGCATGTA
ACCGAGGTTGAACATTGCTTGCGCATTTGATTGAGATTTAGCAT

Last Updated on 06.12.2018 16:11