Sequences from confirmation amplicon


Confirmation sequences for line SALK_054530c and hit At1g07180 are shown.
Line ID SALK_054530c
Locus At1g07180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2205963
Primers used for PCR 81by - LBb3, show primer details
   
Sequence Name 24-K108444-SALK_054530C-81by
GenBank Accession LN484968 [GenBank]
Sequencing primer gene specific primer, 81by: 5'-ACGAGAGCTTTGTATCTCCCAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K108444-SALK_054530C-81by
GGTTCCCCAGCTCCATTTCCTTTGCGCTATTGGCCCGTCCTCCTCCAGCTTTTCCCATCA
CGTTAAACAGATTCGCCAAGTATTTGCCTTCTCTCTCAGCCACCTTCATTGATCAAAGAG
ACTTGTATGTTAAGACTCCGAGTATAGAAAAAAAGTAAATAGATTCTTTTACAACATTAG
AGACCTGTGCAAGTGCAGGAAGTGTTGATTTCCCTGTGCTCTCGAGATAACCGCTACAGT
CACCAATTGCAAACACGTCTTGTACAGAAGGTACACGCATCCACTCATCAATACCAATCC
TATCGAAACAGAGTGATCCAAGATCAGAAAATGTTTTTTTGGAGGGAAAAAAACAGAACA
AGCTTTCTGTTGTGACATTGGAACTAACATACCTTCCACCTGGATCTTTAGGAAAATCAA
GAGACCTCACAAATGAAGATGGACCAACACCAGTTGACCAGACTAAGGGACCGTAAGGAT
ATATTTATGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 08-K108443-SALK_054530C-R210
GenBank Accession LN484482 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K108443-SALK_054530C-R210
TTTGTTACACCATAAATATATCCTTACGGTCCCTTAGTCTGGTCAACTGGTGTTGGTCCA
TCTTCATTTGTGAGGTCTCTTGATTTTCCTAAAGATCCAGGTGGAAGGTATGTTAGTTCC
AATGTCACAACAGAAAGCTTGTTCTGTTTTTTTCCCTCCAAAAAAACATTTTCTGATCTT
GGATCACTCTGTTTCGATAGGATTGGTATTGATGAGTGGATGCGTGTACCTTCTGTACAA
GACGTGTTTGCAATTGGTGACTGTAGCGGTTATCTCGAGAGCACAGGGAAATCAACACTT
CCTGCACTTGCACAGGTCTCTAATGTTGTAAAAGAATCTATTTACTTTTTTTCTATACTC
GGAGTCTTAACATACAAGTCTCTTTGATCAATGAAGGTGGCTGAGAGAGAAGGCAAATAC
TTGGCGAATCTGTTTAACGTGATGGGAAAAGCTGGAGGAGGACGGGCCAATAGCGCAAAG
GAAATGGAGCTTGGGGAACCATTTGTGTATAAACATCTCGGAAGTATGGCTACTATTGGG
AGATACAAAGCTCTC

Last Updated on 06.12.2018 16:11