Sequences from confirmation amplicon


Confirmation sequences for line SALK_057452 and hit At4g17486 are shown.
Line ID SALK_057452
Locus At4g17486 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9751273
Primers used for PCR 14ck - LBb3, show primer details
   
Sequence Name 28-K108444-SALK_057452C-14ck
GenBank Accession LN485079 [GenBank]
Sequencing primer gene specific primer, 14ck: 5'-ACTTTTCTCGATCATTTTTGCAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K108444-SALK_057452C-14ck
AGCTCCGTTTCCTTCGTCTTCCTCGTCTCTCTCGATCTCCCTTAAACGATTGTTGTACAT
GAAAACGCACGCGCCCAATTCCCTTTATGTTTCGTGGCTTTCCTGATTTGAAATTCGAGC
GATTGGATAGATTTTTCTTTTAGAGGATTAGATTTTTGATTGTATGTGTAATTGCTACTG
TGTGAATCGAATCCTTGGATCTGTTCTCGTTAGTCGCCAAAGAAGAAGTTGTATACATGT
TTGATTGTTTATGTTCGAATTGCTTGAATTCCAATTATTTCGATCTAAGTTATGGAACAT
TTGATTGTTACTTCTGGTGTTTATATGATGAATATGACCATCAGTTTTTTCTTTTT
   
Sequence Name 12-K108443-SALK_057452C-R210
GenBank Accession LN484604 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K108443-SALK_057452C-R210
GTCATTTGTTACCCACAATATATCCTGAACATGAGCTTGAGCTCAATGTAGGCACCCACA
TTTTACACACAATATCCCAATTCAAAGTCACGATCGAACCATTCCTTTCTTCCTAAACCT
CTGCAACACTAAAAAAAAAAAAAACGGGGGGTCATATTCATCATAAAAACCCCAAAAGTA
AAAATCAAAGGTCCCAAAATTTAAATCAAAAAAATGGGAATTCAAGCAATTCAAACAAAA
ACAATCAAACTTGTATACAATTTTTTCTTGGGGGACAAACGAAAACAAATCCAGGGATTC
AATTCCCACGGTACCATTTACCCATAAAATCAAAAATCTAATCCTCTAAAAAAAAAATTT
ACCCAATCCCTCAAATTTCAAATCGGGAAACCCCCAAAACATATTTGAATTGGGGCGCGG
GGTTTTTCTTGTACAAAATTCGACCAAAAAAAATCGAAAAAAACGAGGAAAACAAAGGAA
CCGGACCTTCCTTGAAATTCAACCAAAAGGGAATGGGTGGGGAAAAAAACTGCAAAAATG
ATCGAAAAAAAA

Last Updated on 06.12.2018 16:11