Sequences from confirmation amplicon


Confirmation sequences for line SALK_058044c and hit At3g13810 are shown.
Line ID SALK_058044c
Locus At3g13810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4546942
Primers used for PCR 31de - LBb3, show primer details
   
Sequence Name 06-K109680-SALK_058044C-31de
GenBank Accession LN484424 [GenBank]
Sequencing primer gene specific primer, 31de: 5'-ATTCATCAATCTGCTTCTCATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109680-SALK_058044C-31de
CCACCACCATAACGTCTCTTCTTCTTCATCTTCCTCCCACAACCACAACATCATCAATAG
CCTTCACTTCGACACCAACAATGGTAATACTAATAATAGTAACAACAGCAACAACCATCT
CCATACATTTCCCATGAAGAAAGAGCAACAAAGCAATGACCATATCATGAATTACCACCA
TAGTATCATCCCTCCTTGGCTTGCTCCTCAGCCTCACGCACTCACATCTTCAAACCCTAA
CCCTAGTAATGGTGGAGGAGGAGGAGGAAGTTTGTTCTCTCTTGCTTCTCCGGCTATGTC
AGCCACCGCATTGCTCCAGAAAGCAGCCCAAATGGGCTCTACAAAGACACCTCCTTTACC
ACCAACCACGGCCTACGAGAGGTCAACTCATAATAACAACCTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCCGCAAA
   
Sequence Name 22-K109679-SALK_058044C-R210
GenBank Accession LN484909 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109679-SALK_058044C-R210
TGAGTTGACCTCTCGTAGGCCGTGGTTGGTGGTAAAGGAGGTGTCTTTGTAGAGCCCATT
TGGGCTGCTTTCTGGAGCAATGCGGTGGCTGACATAGCCGGAGAAGCAAGAGAGAACAAA
CTTCCTCCTCCTCCTCCACCATTACTAGGGTTAGGGTTTGAAGATGTGAGTGCGTGAGGC
TGAGGAGCAAGCCAAGGAGGGATGATACTATGGTGGTAATTCATGATATGGTCATTGCTT
TGTTGCTCTTTCTTCATGGGAAATGTATGGAGATGGTTGTTGCTGTTGTTACTATTATTA
GTATTACCATTGTTGGTGTCGAAGTGAAGGCTATTGATGATGTTGTGGTTGTGGGAGGAA
GATGAAGAAGAAGAGACGTTTATGGTTGGTTGTGTTTGATGATGGTGATGAGGATGAGAA
GCAGATTGATGAA

Last Updated on 06.12.2018 16:11