Sequences from confirmation amplicon


Confirmation sequences for line SALK_060839 and hit At1g17450 are shown.
Line ID SALK_060839
Locus At1g17450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5992291
Primers used for PCR 33ux - LBb3, show primer details
   
Sequence Name 21-K118412-SALK_060839C-33ux
GenBank Accession LN484897 [GenBank]
Sequencing primer gene specific primer, 33ux: 5'-TGCCAAATAATTTTCTTTTTGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118412-SALK_060839C-33ux
CACTCTTATTTCTTTGATGTTTCAGCTTGATTTCGGTTTTAAAGCTTTCTCATATCTGGT
CCACAAACAGAGACTGACCTGAATGTTCTCTTTTTTCCTCTTGTTTTACTGGGGTGACGT
AGCTAATAGTTTTTGGTTGCGTACTCCAGGTTATGCATGTCTATCATGCGAAGCATGGAA
GACGCGTGAAATCCAAATCAAAAGATAAACATTTAGCTATAGATAATAGCTCTTCTTCAT
CTTCTGGGAAAAGAAAAAGAGGAACTCTCGTAAAGACTACAGGAGAAGGTGTCAGATCCA
TAATAGTAGACGGAGAAAAGGTTCTGAATTCCGATGCCATTGATGCCTCAAATAGTGAGA
AGTTTCTAAATTCATTAGAAGAGCATCAGGAGCATAATCTACAAGAAAATTCAGAGATAA
GGGATCTTACTGAAGATGAAGGCCAGTGCTCTTCTATTATCAACCAGTACGCAAGTTCAA
AGACCACATCATCACAAAGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 05-K118411-SALK_060839C-R210
GenBank Accession LN484413 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118411-SALK_060839C-R210
CATTTGTTTCACCACAATATATCTTTGTGATGATGTGGTCTTTGAACTTGCGTACTGGTT
GATAATAGAAGAGCACTGGCCTTCATCTTCAGTAAGATCCCTTATCTCTGAATTTTCTTG
TAGATTATGCTCCTGATGCTCTTCTAATGAATTTAGAAACTTCTCACTATTTGAGGCATC
AATGGCATCGGAATTCAGAACCTTTTCTCCGTCTACTATTATGGATCTGACACCTTCTCC
TGTAGTCTTTACGAGAGTTCCTCTTTTTCTTTTCCCAGAAGATGAAGAAGAGCTATTATC
TATAGCTAAATGTTTATCTTTTGATTTGGATTTCACGCGTCTTCCATGCTTCGCATGATA
GACATGCATAACCTGGAGTACGCAACCAAAAACTATTAGCTACGTCACCCCAGTAAAACA
AGAGGAAAAAAGAGAACATTCAGGTCAGTCTCTGTTTGTGGACCAGATATGAGAAAGCTT
TAAAACCGAAATCAAGCTGAAACATCAAAGAAATAAAGAGTTGGCATTATCTCTAAGATC
AAGGACAAAAAGAAATTATTTTTGGCA

Last Updated on 06.12.2018 16:11