Sequences from confirmation amplicon


Confirmation sequences for line SALK_061645c and hit At1g23460 are shown.
Line ID SALK_061645c
Locus At1g23460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8328413
Primers used for PCR 26di - LBb3, show primer details
   
Sequence Name 07-K114130-SALK_061645C-R210
GenBank Accession LN484465 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114130-SALK_061645C-R210
TTATTTGGTTTCGTTCAACAATCTGTTTTTGTTTTCCCCTGCAGGCACTAACTATAGAAT
CTAGTTCAGGTGTGAAAGTAAGTGGCTTGACAATCCAGAACAGCCAGCAGATGAATTTCA
TCATAGCAAGGTCAGATTCAGTTCGAGTCTCCAAGGTTATGGTCTCTTCTCCAGGAGATA
GTCCCAACACTGATGGAATCCATATCACTGGATCTACCAATGTTATCCTTCAAGACTGTA
AAATCGGCACAGGTTTTTTTTCTTTTGACCAAACCTTTATGCAAATGAAACATTTTACAC
TTCGACTACGGTTGTAATAATCCCTTAAGACTGGATTAAACAGGGGATGATTGTGTCTCG
ATTGTGAATGCCAGCTCGAATATAAAGATGAAGAACATCTATGGTGGACCTGGACATGGA
ATCAGGTACATCATCA
   
Sequence Name 17-K114131-SALK_061645C-26di
GenBank Accession LN484750 [GenBank]
Sequencing primer gene specific primer, 26di: 5'-TTGATGATGTACCTGATTCCATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114131-SALK_061645C-26di
ATTCGAGCTGGCATTCCAATCGAGACACAATCATCCCCTGTTTAATCCAGTCTTAAGGGA
TTATTACAACCGTAGTCGAAGTGTAAAATGTTTCATTTGCATAAAGGTTTGGTCAAAAGA
AAAAAAACCTGTGCCGATTTTACAGTCTTGAAGGATAACATTGGTAGATCCAGTGATATG
GATTCCATCAGTGTTGGGACTATCTCCTGGAGAAGAGACCATAACCTTGGAGACTCGAAC
TGAATCTGACCTTGCTATGATGAAATTCATCTGCTGGCTGTTCTGGATTGTCAAGCCACT
TACTTTCACACCTGAACTAGATTCTATAGTTAGTGCCTGCAGGGGAAAACAAAAACAGAT
TGTTGAACGAAACCAAATAAGCAGCCATTTGTAAGGAATAATAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA

Last Updated on 06.12.2018 16:11