Sequences from confirmation amplicon


Confirmation sequences for line SALK_066378c and hit At2g33480 are shown.
Line ID SALK_066378c
Locus At2g33480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14181357
Primers used for PCR 22md - LBb3, show primer details
   
Sequence Name 11-K114334-SALK_066378C-22md
GenBank Accession LN484589 [GenBank]
Sequencing primer gene specific primer, 22md: 5'-CAAGAACACTCTACACAAAACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114334-SALK_066378C-22md
TTTAACCATTAAATACCTTTTTAGCCAGTTTTTATCCACTGATTGATGAATCTAATTTAT
TTGAATTTGATTCTCTTACATATAATGAATCTTGTTGTGAATCAACAAGACGATATTCAT
GAAGAACCCAGTTAGTTCTTGTTCCGTTTGGTGGTTTACCTTTGTAGAAAACAAGAGTTT
TCTTCATCCCCACGACAAGCTTCTTCTTACCGATCTGCTTATCGAGACCAGTCGCTTTCC
AATAACCCGAACCGGTAGACCGGTTCGACCGGTTTCCGTTCGGGTATTTAGCTTCCCTCG
TGCTAAAAAAATACATCTCTGATTCACAATCACCTGTGATCATCATGCATATACACCATC
AAATATTGAATTTGAATGAAACCCTAGAGTAGATTTTTCAATTTCTGTACCTGGTAAATC
CCATGGATCGGATTTACAAACATCGGTTTCCGGTATTACAGAAGCTGGTAAGGGTAAACC
GGTTACTTTTCGACGTANATATTGAACCACTAACACATTGCGGACAACAGCTGCGGACGT
TTATGGACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACT
GGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGG
CCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAAAATCCTGT
TTGAT
   
Sequence Name 01-K114333-SALK_066378C-R210
GenBank Accession LN484287 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114333-SALK_066378C-R210
CCGCAGCTGTTGTCCGCAATGTGTTAGTGGTTCAATATTTACGTCGAAAAGTAACCGGGT
TTACCCTTACCAGCTTCTGTAATACCGGAAACCGATGTTTGTAAATCCGATCCATGGGAT
TTACCAGGTACAGAAATTGAAAAATCTACTCTAGGGTTTCATTCAAATTCAATATTTGAT
GGTGTATATGCATGATGATCACAGGTGATTGTGAATCAGAGATGTATTTTTTTAGCACGA
GGGAAGCTAAATACCCGAACGGAAACCGGTCGAACCGGTCTACCGGTTCGGGTTATTGGA
AAGCGACTGGTCTCGATAAGCAGATCGGTAAGAAGAAGCTTGTCGTGGGGATGAAGAAAA
CTCTTGTTTTCTACAAAGGTAAACCACCAAACGGAACAAGAACTAACTGGGTTCTTCATG
AATATCGTCTTGTTGATTCACAACAAGATTCATTATATGTAAGAGAATCAAATTCAAATA
AATTAGATTCATCAATCAGTTGGATAAAAACTTGGCTAANAAGTGTATTTTAATGTGTTT
TTAAAAGGGACAGAACATGAATTGGGTTTTGTGTAAGNTGGTTCTTG

Last Updated on 06.12.2018 16:11