Sequences from confirmation amplicon


Confirmation sequences for line SALK_067559c and hit At4g25620 are shown.
Line ID SALK_067559c
Locus At4g25620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13067535
Primers used for PCR 17ii - LBb3, show primer details
   
Sequence Name 33-K112623-SALK_067559C-17ii
GenBank Accession LN485186 [GenBank]
Sequencing primer gene specific primer, 17ii: 5'-CTGGTTCAGGTACATCTTCTCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K112623-SALK_067559C-17ii
GCGAACTCCAAGTTTCTTGGTTTTGAGCACTTCACAGCGCGTAAATGGGGATCAAGATTC
GGTTCTGGATCCATCACACCTGCTGGACAAGGTTCAAGGTTGGGTTCAGGTGCTTTGACT
CCTGATGGCTCAAAGCTAACTTCTGGTGTAGTGACACCAAATGGTGCAGAGACTGTTATA
AGAATGAGTTATGGGAATCTCACACCACTTGAAGGCAGTCTTTTGGATAGTCAGATCTCT
GAGGTTGCGTCTTTAGCCAATTCGGACCACGGGTCGTCAAGGCATAATGATGAAGCTCTC
GTGGTTCCTCACAGAGTTTCTTTCGAGTTGACTGGTGAAGACGTTGCACGGTGTCTTGCA
AGCAAGCTAAACCGTTCCGGTTCACATGAAAAAGCAAGCGGCGAACATTTAAGACCAAAC
TGTTGTAAAACGTCGGGAGAAACAGAGAGCGAACAGAGTCAGAAGCTAAGATCGTTTTCT
ACAGGCTCTAACAAAGAATTCAAGTTTGATAGCACCAATGAAGAGATGATAGAGAAAATT
CGATCGGAGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 09-K112622-SALK_067559C-R210
GenBank Accession LN484521 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112622-SALK_067559C-R210
GTCATTTGTTACACCACTCCGATCGAATTTTCTCTATCATCTCTTCATTGGTGCTATCAA
ACTTGAATTCTTTGTTAGAGCCTGTAGAAAACGATCTTAGCTTCTGACTCTGTTCGCTCT
CTGTTTCTCCCGACGTTTTACAACAGTTTGGTCTTAAATGTTCGCCGCTTGCTTTTTCAT
GTGAACCGGAACGGTTTAGCTTGCTTGCAAGACACCGTGCAACGTCTTCACCAGTCAACT
CGAAAGAAACTCTGTGAGGAACCACGAGAGCTTCATCATTATGCCTTGACGACCCGTGGT
CCGAATTGGCTAAAGACGCAACCTCAGAGATCTGACTATCCAAAAGACTGCCTTCAAGTG
GTGTGAGATTCCCATAACTCATTCTTATAACAGTCTCTGCACCATTTGGTGTCACTACAC
CAGAAGTTAGCTTTGAGCCATCAGGAGTCAAAGCACCTGAACCCAACCTTGAACCTTGTC
CAGCAGGTGTGATGGATCCAGAACCGAATCTTGATCCCCATTTACGCGCTGTGAAGTGCT
CAAAACCAAGAAACTTTGGAGGTTCGCCGATACGAAACTCGATGATGGAGCATTTCCCTG
GGTAAGGAGAAGATGTACCTGAACCAGA

Last Updated on 06.12.2018 16:11