Sequences from confirmation amplicon


Confirmation sequences for line SALK_070257c and hit At5g06000 are shown.
Line ID SALK_070257c
Locus At5g06000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1808876
Primers used for PCR 24hd - LBb3, show primer details
   
Sequence Name 70-K112564-SALK_070257C-24hd
GenBank Accession LN486004 [GenBank]
Sequencing primer gene specific primer, 24hd: 5'-AACTAACGAACTTAATAAGCGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K112564-SALK_070257C-24hd
GATACAAAAACAAAAAGCTTCGGTGGGGAGAAATTGACGAAGAAGAAGGCGACTATGATT
TCCTTCTTCCGCCTAAGCAGATGATTAGTCCGGACCAGAACGGTGTAAAGAAGGTGATTG
AGTACAAATTCAACGAAGAGGACAAAAAGGTCAAAATCACTACCACGACCCGTGTTCAGA
AGCGAGCTCTCACCAAACAAGCCGTGGAGCGACGGAGCTGGAATAAGTTCGGAGACGCAG
CTCATGAAGAATCCAGTAGTTACCTCACAATGCGTTCAACAGAGGATATCATCTTGGAAC
GAATTAGAGCTCCTGGTAAAATCTTCAACTTTGCATTTTAGTTTGTTAGGATTCGTTGTT
ATTATGCTCTGTTTTGATTAGGGTGGCTAATTAGCCAGTCACATGTTAGCTTTATGGCTA
CTGTTTGTGTGAATGGTCAGTAAGCACACCTATAGCAATGTCGTTGGTGATTCGTTTCAA
CACTAGAACAGGATTTAACCCAGTTTTTGGTTATGGATCATCAACTATATCTTTGGTTCA
AGTTTTTTTTTTGTAACTGTGTAACATAACAAAGGTGCCCAATTAGCCAATCACATATAA
GCCATATATGGCTGCTTCTTTCTGTGATTGGTCAGTATGTCACCAATATAAGTATCTGGT
TGGTAGCCTTTTCTGAATGTGGACAAATTCTGTGTAAACAAATTGACGCTAAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCACCAGGCGAAAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 54-K112563-SALK_070257C-R210
GenBank Accession LN485606 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K112563-SALK_070257C-R210
TTTGTTACCAGAATTTGTCCCATTCAGAAAAGGCTACCAACAAGATACTTATATTGGTGA
CATACTGACCAATCACAGAAAGAAGCAGCCATATATGGCTAATATGTGATCGGCTAATTG
GGCACCTTTGTTATGTTACACAGTTACAAAAAAAAAACTTGAACCAAAGATATAGTTGAT
GATCCATAACCAAAAACTGGGTTAAATCCTGTTCTAGTGTTGAAACGAATCACCAACGAC
TTTGCTATAGGTGTGCTTACTGACCATTCACACAAACAGTAGCCATAAAGCTAACATGTG
ACTGGCTAATTAGCCACCCTAATCAAAACAGAGCATAATAACAACGAATCCTAACAAACT
AAAATGCAAAGTTGAAAATTTTACCAGGAGCTCTAATTCGTTCCAAAATGATATCCTCTG
TTGAACGCATTGTGAGGTAACTACTGGATTCTTCATGAGCTGCGTCTCCAAACTTATTCC
AGCTCCGTCGCTCCACGGCTTGTTTGGTGAGAGCTCGCTTCTGAACACGGGTCGGGGTAG
TGATTTTGACCTTTTTGTCCTCTTCGTTGAATTTGTACTCAATCACCTTCTTTACACCGT
TCTGGTCCGGACTAATCATCTGCTTAGGCGGAAGAAGGAAATCATAGTCGCCTTCTTCTT
CGTCAATTTCTCCCCACCGAAGCTTTTTTGTTTTTTGTATCGTATCAATCGCCATTTTTC
TCAAATTTAGATATGCCGCTAATTAA

Last Updated on 06.12.2018 16:11