Sequences from confirmation amplicon


Confirmation sequences for line SALK_071365 and hit At3g59540 are shown.
Line ID SALK_071365
Locus At3g59540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21996305
Primers used for PCR 14jd - LBb3, show primer details
   
Sequence Name 23-K113259-SALK_071365C-14jd
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 14jd: 5'-TAACGACTGCTTTTTCTCCAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K113259-SALK_071365C-14jd
ACATTGTACCCCAAAAAAACTCCTTTCCCATCATATGTTGAAAAATGTCTTGCTCTG
   
Sequence Name 06-K113258w-SALK_071365C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113258w-SALK_071365C-R210
AATTTGTCAGGTGCTCAAGGTACCTCTACACACTATGTGCTCTTTACCAAGAGAAGGCCG
ATAAGTTGAAGCAGTCTCTTCCTCCAGGTTCACTATCTCTCTCTCTCTCTCTCTGTGACT
CAGCCCAAACATTACACACTTGAAACCTCTTATCTCATAACATTTGTTAGATTGGATAGA
TGTTCCTAGCTTTGAGTAATTCATCACTTGAAAGTTCTAGTGTCATAATATTGTTGGATT
TTGGTTTTTGAATTCGAATAGTCTTGTGGTTAATCTACTTTAATTCAGACACTTTTAATT
GGCATGTTTAGGTCACAAGTTATTTTTGGTTGGTCCATGAACGCATGCGTCTATCTCTAT
CTAAGTTGAATTTGCCAATTGTTTATTTCAGGTTTGAGTGTGCAAGACCTTTGAAGATCT
TATGAAATCAGGATCTATCATGGTTTTATATACCCTTGTCTTAGATGATTTTGTTTTTTC
CCTTTCTGCTGTCTTTTGAATGACTATGATTCAGACTTCCGTTGAGTTTCTTTCTTATAA
CTTCAAGACTAAATTATGAGTTGGATTTGTATTTTTTTTTGCTATCAACTTGGTTAGTAA
TATATATGGCCCCAACAGTTTGGAGTTTTGGATAGTTGTGCTTTGAGAAGAAAGCAGACA
CCACAGATTTCATAAAATGTTCATTGCTATGTGATGCCAAACAAACTACAGAAAAAGTTA
CTGAAAAAAGAGTAAAACGTCGTAATGGAGATGATTGTAACGTCATCATTTTGGAGAA

Last Updated on 06.12.2018 16:11