Sequences from confirmation amplicon


Confirmation sequences for line SALK_071663c and hit At1g75400 are shown.
Line ID SALK_071663c
Locus At1g75400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28298628
Primers used for PCR 50cy - LBb3, show primer details
   
Sequence Name 50-K109566-SALK_071663C-50cy
GenBank Accession LN485502 [GenBank]
Sequencing primer gene specific primer, 50cy: 5'-TGAAAGACAGAAAGGTTCCTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K109566-SALK_071663C-50cy
CATGTTCGCCACAATGGAGTTTTCGACGTGATAATAGGAGGCGAGTGGCTGATGAGATTG
AGGGTTCACCTTATTACAGTCCTTATGTTGCGAGTAGAGGTATTAGTATGGATAAGATGT
CATTAGGCTCTGAGAGAGGAACTTTATCTGAAGGAGGAACCCCTCCTGATGGACATTTAG
GGACTCCTGCTTCTCAGAAATCAGCAACTCCGGAAATGAGCACCAATTCCATGGTTCCAC
CTTCTTCAGGTAATTCTTTTTTTGAGACAATGACACTATTGGGAGCTAATTCTGACTGTA
ATCTAACTCTTTGCTTTGAGTCGTTTTAATTTTTTCCTTGAATCTTTTCGTTCCACCAAC
GTGTATAAGTAAAGCTTGCTAGTTTTCATGATCTCTTCACGGTGTTGAGTAGATATAGTT
GACATAGGGATTTTTAAGTTGAACGTTAATGACTATATTCCTTTTTGTCACCAAGAACCA
TGTTATTGTAATGAATTAGTCTTGCGTATCGTATTCCTGCTGTGTCTTATGTTATGCTTT
TCTGTGCGGCAGGTTCATCCTTGGCAAGCAGTGATCTCATTGAGGTGAGATGATCGACTT
GACTCTAGTGTTCCTCTCGTGGAAGCTTTCTTTTCTTTCCTTCGGTGTATCTTTA
   
Sequence Name 41-K109565-SALK_071663C-R210
GenBank Accession LN485307 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K109565-SALK_071663C-R210
GTCATTATGATGGCGTAGCCAAAGGGCAATGTGTTACCTTCCTTCTGAGATTGAGTAATT
ATTAATTGGGGATTTCAGTCCTAAAATTTGACTATCAGAAACCTGCCTAAACAACTGATG
CCCAGGTGAACGCCGAGCCCGTCTTGATGGTGTGGATTTAGGAGGAAGCAAACGCGTGTG
AGCCGATGAAAGATCACTAACTGGATTGGGAAGAGAAGGCGTGGAGAACAAAGGCTTCGA
TTGAGCTGAAGGGACAATTCCAGGTGAGTCAACTAAGTTCTTCACCTGAAACAATGAAGA
TCCAACAAGGGTTAAAGATACACCGAAGGAAAGAAAAGAAAGCTTCCACGAGAGGAACAC
TAGAGTCAAGTCGATCATCTCACCTCAATGAGATCACTGCTTGCCAAGGATGAACCTGCC
GCACAGAAAAGCATAACATAAGACACAGCAGGAATACGATACGCAAGACTAATTCATTAC
AATAACATGGTTCTTGGTGACAAAAAGGAATATAGTCATTAACGTTCAACTTAAAAATCC
CTATGTCAACTATATCTACTCAACACCGTGAAGAGATCATGAAAACTAGCAAGCTTTACT
TATACACGTTGGTGGAACGAAAAGATTCAAGGAAAAAATTAAAACGACTCAAAGCAAAGA
GTTAGATTACAGTCAGAATTAGCTCCCAATAGTGTCATTGTCTCAAAAAAAGAATTACCT
GAAGAAGGTGGAACCATGGAATTGGTGCTCATTTCCGGAGTTGCTGATTTCTGAGAAGCA
GGAGTCCCTAAATGTCCATCAGGAGGGGTTCCTCCTTCAGATAAAGTTCCTCTCTCAGAG
CCTAATGACATCTTATCCATACTAATACCTCTACTCGCAACATAAGGACTG

Last Updated on 06.12.2018 16:11