Sequences from confirmation amplicon


Confirmation sequences for line SALK_072621c and hit At3g52450 are shown.
Line ID SALK_072621c
Locus At3g52450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19441051
Primers used for PCR 20gg - LBb3, show primer details
   
Sequence Name 26-K112129-SALK_072621C-20gg
GenBank Accession LN485032 [GenBank]
Sequencing primer gene specific primer, 20gg: 5'-TCGAGCTTTTAATGGACGATACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K112129-SALK_072621C-20gg
GCGATGGTGGTTCTTGATATGTTATGTCAGTGCGCAGAAGGAAGAGCTGAGTTCTTGAAT
CACGGTGCCGCTATTGCGGTTGTGTCTAAGAAGATATTGAGGGTCTCACAAATAACTAGT
GAAAGGGCAGTTAGGGTTTTGCTTTCGGTTGGGAGGTTCTGCGCGACACCCTCTTTGTTG
CAGGAGATGTTGCAATTGGGAGTTGTAGCAAAGCTGTGTCTGGTACTTCAAGTTAGCTGT
GGTAACAAGACTAAAGAAAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCG
   
Sequence Name 10-K112128-SALK_072621C-R210
GenBank Accession LN484550 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112128-SALK_072621C-R210
ACAGCTACTTGAAGTACCAGACACAGNCTTTGCTACAACTCCCAATTGCAACATCTCCTG
CAACAAAGAGGGTGTCGCGCAGAACCTCCCAACCGAAAGCAAAACCCTAACTGCCCTTTC
ACTAGTTATTTGTGAGACCCTCAATATCTTCTTAGACACAACCGCAATAGCGGCACCGTG
ATTCAAGAACTCAGCTCTTCCTTCTGCGCACTGACATAACATATCAAGAACCACCATCGC
CATCTCTGAATTCCTTCTCTCTGATGAGAAAGTATCGTCCATAAAAAGCTCG

Last Updated on 06.12.2018 16:11