Sequences from confirmation amplicon


Confirmation sequences for line SALK_079505c and hit At5g29000 are shown.
Line ID SALK_079505c
Locus At5g29000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:11023253
Primers used for PCR 13kw - LBb3, show primer details
   
Sequence Name 59-K114145-SALK_079505C-R210
GenBank Accession LN485720 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K114145-SALK_079505C-R210
GCGTCATATGTGTATTCAGAGCTTCAAAACAGCCTTAGGGGTGGCTCCTATGATGGAAAT
TATAGAAGAATCAGTGTACGGTTAACATCCTTGAGCAATTATTTTCAGGAACAATAGAAG
GAGAAAGCTTACGCACGTTCACTACCACCAAGCTGATTAACAGCTTCAACGAATGCCTCG
TGAAGTTCTGGTGTCCAACGCATACGTTGTTTAGATGTTGCTACACTAGATGATGAGTTT
CTGCCGCTGAGCTGGTCCTCTGATGAAACCATCTGTTGCTGCTGGTTAGCTGTTATATCT
AGCCTTGGTACATCCAAAAATGGTGTAGGTATCTGATACCAATTATGCAAAACACTGATT
AAATAACATAAGGACAACCAACAAACATAGAGACCATATATGGCTAGAGAAACTGAACCT
CTGAATTTGGATTGTGGGAACTTGAATCTCCNAGAAG
   
Sequence Name 70-K114146-SALK_079505C-13kw
GenBank Accession LN486009 [GenBank]
Sequencing primer gene specific primer, 13kw: 5'-AAATTGGTCTGAACTTCTTGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K114146-SALK_079505C-13kw
TTTCTCTGCCAATATGGTCTCTATGTTTGTTGGTTGTCCTTACGTTATTTAATCAGTGTT
TTGCATAATTGGTATCAGATACCTACACCATTTTTGGATGTACCAAGGCTAGATATAACA
GCTAACCAGCAGCAACAGATGGTTTCATCAGAGGACCAGCTCAGCGGCAGAAACTCATCA
TCTAGTGTAGCAACATCTAAACAACGTATGCGTTGGACACCAGAACTTCACGAGGCATTC
GTTGAAGCTGTTAATCAGCTTGGTGGTAGTGAACGTGCGTAAGCTTTCTCCTTCTATTGT
TCCTGAAAATAATTGCTCAAGGATGTTAACCGTACACTGATTCTTCTATAATTTCCATCA
TAGGAGCCACCCCTAAGGCTGTTTTGAAGCTCTTGAATAACACATATTGACGCTTAGACA
ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTG
AGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGT
CCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGT

Last Updated on 06.12.2018 16:11