Sequences from confirmation amplicon


Confirmation sequences for line SALK_079981c and hit At3g45310 are shown.
Line ID SALK_079981c
Locus At3g45310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16628738
Primers used for PCR 07jd - LBb3, show primer details
   
Sequence Name 08-K113376-SALK_079981C-07jd
GenBank Accession LN484494 [GenBank]
Sequencing primer gene specific primer, 07jd: 5'-TACATGTGGTAACACTCCAATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K113376-SALK_079981C-07jd
CATTACCAACGTTTAGTAAGTAAACCGGGTTTTTTTGGTGGCAACAGGATGTAAACCATG
CAGTGTTAGCAGTTGGTTATGGAGTTGAAGACGACGTCCCGTACTGGCTTATAAAGAACT
CATGGGGAGGTGAATGGGGAGACAATGGCTACTTCAAGATGGAAATGGGAAAGAACATGT
GTGGTAAGTTCTTTTAACCAGCTTTGATTTCGGTTTAGATCATTAAGTTAGCATAGAAGA
AATAAAATCGAATCGGTTTGGTTCGGTTAATTCTTCTCTATCCCATTGATTTTTTTCCTT
TGATTTTGGCAGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 04-K113375-SALK_079981C-R210
GenBank Accession LN484371 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113375-SALK_079981C-R210
TTTGTTACACCTGCCAAATCAAAGGAAAAAATCAATGGGATAGAGAAGAATTAACCGAAC
CAAACCGATTCGATTTTATTTCTTCTATGCTAACTTAATGATCTAAACCGAAATCAAAGC
TGGTTAAAAGAACTTACCACACATGTTCTTTCCCATTTCCATCTTGAAGTAGCCATTGTC
TCCCCATTCACCTCCCCATGAGTTCTTTATAAGCCAGTACGGGACGTCGTCTTCAACTCC
ATAACCAACTGCTAACACTGCATGGTTTACATCCTGTTGCCACCAAAAAAACCCGGTTTA
CTTACTAAACGTTGGTAATTGTAATATTTTTGTGGTTTACAGCAGAAAAGTACTCACCAT
TGGAGTGTACCCACATGT

Last Updated on 06.12.2018 16:11