Sequences from confirmation amplicon


Confirmation sequences for line SALK_081127c and hit At5g44720 are shown.
Line ID SALK_081127c
Locus At5g44720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18043352
Primers used for PCR 37cv - LBb3, show primer details
   
Sequence Name 81-K111509-SALK_081127C-37cv
GenBank Accession LN486233 [GenBank]
Sequencing primer gene specific primer, 37cv: 5'-ATCAAACAAGACCCTTTTCTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K111509-SALK_081127C-37cv
AGAACGAAATGAGAGTGAAACTGTAGTCTTCATGCCAACAAAAAAAAAAAAAAAAAAACT
TAAGGGGCCCCCCCCATAAACCCCAAAAAATACCACCTCCCCAAAATTGGTCCCTTTCCC
TCCCAACCAAACAAAAACATTTCCCAAAAAATTGGTCCTTTTCCCACCCAAAAACCAAAA
AAGGCAGAACCCTTTCTGGTACCACCAACCCCGAACCATGGAGGGGGAAAAACCTAATGG
TCCAGGCCACCAAAAAAGGGGAAACTTAAGGGGTCCCCCCCCATTTACCCCAATAAAAAC
CATTTTCGAAAACATTTGGTCCCTTTTCCGGAAAATTTGGTGGACAATTGGGGGGGAAAA
AATAAGGGATATTTGGGGGGGAAAACAATTGGCCCCTAAAACAACTAAAAAACACTTGGG
GAACTTTTTAAAGGAACGGGGGGGGTTTTCCTTTCCCCCAGGGAAAGGGGCAACACCGAA
TGGCCCTCCCCCCCCGGGCCCTAAAAAATTGGCACCAGGGGGCCCACCCGGGTTGGCCCC
ACCGGGCAAAAACCCGGTTGAAGGGGGGTCCCAAA
   
Sequence Name 67-K111508-SALK_081127C-R210
GenBank Accession LN485927 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K111508-SALK_081127C-R210
CATTTGTTTCACCACAATATATCCTTAATTTGTTCACCACAATTTGTCAACAAATTTTCC
GAGAAAGTGAACAAATTGTCTCAGAAATTGCTATTAATAGGGTAAAATGAGAGTGAACAC
TTAAAGTCTCCACTTTTATGTTGGCATGAACATTTAGATTTTTCACTCTCATTGTTACGG
GATCGATGATACTAGAAAGGGTCTTGCTTTATTAGTTTATTGGTTGGAAAATGAACAAAT
TATCTGGAAATTGTTCTTGTTTGATTGGATGGAAAGTGAACAATTTCTGGGAAGATGATA
TTATTTGGGTATAATGAGAGTGACCACTAAAGTTTTTCTCTTTTTTTTTTTGTGGGCATA
AAAACAACGTTTCCCCCCCAATTTCTTTTCCTTTAAATTTAAGGGGTTCAAAAATACCAA
AAAGGGGTTTTGTTTAAA

Last Updated on 06.12.2018 16:11