Sequences from confirmation amplicon


Confirmation sequences for line SALK_082287c and hit At3g11030 are shown.
Line ID SALK_082287c
Locus At3g11030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3458388
Primers used for PCR 14vt - LBb3, show primer details
   
Sequence Name 73-K118240-SALK_082287C-14vt
GenBank Accession LN486092 [GenBank]
Sequencing primer gene specific primer, 14vt: 5'-CCACTTTACAGAGAATCCGAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118240-SALK_082287C-14vt
CCCGATAGTGATTATCAGTCATGGAGATGGCGACCTGATTCCTGCTCACTTCCTTCGTTA
GTCTCTCTCTCCGTTCGATTTTGAATGCGATTCGGTGATTGATCTTCAGATCGGATTTGC
TCTGTGGTTGTTGAATGTGTTAATAGGTTTAATGCGACGGTGATGTTGGAGAGCTTAAGA
GGGAAGAAGATGATGTTCGTTGGAGATTCGTTGAACAGAGGAATGTATGTTTCTCTCATC
TGTCTTCTTCATTCACAAATCCCAGAGAACTCCAAATCCATGGATACTTTCGGATCACTC
ACCGTCTTCTCTCTCAAGGTAAGTTTGGTTCAGATTTAGAAAGTCTTGCGGAAGCCATTG
AAGTTGCTTTGTAGAATCTGATGTTACAATTCATAGAGATCTGAGTATGAGGATGACAAA
GATTAAGCTTAGATGTGAAAACTGTTGGCTTGGTTAGGATTCTTATAGATTCTTGTGGTA
TGTTTATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 50-K118239-SALK_082287C-R210
GenBank Accession LN485519 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118239-SALK_082287C-R210
CATTTGTTTACCCACAATAAACATACCACAAGAATCTATAAGAATCCTAACCAAGCCAAC
AGTTTTCACATCTAAGCTTAATCTTTGTCATCCTCATACTCAGATCTCTATGAATTGTAA
CATCAGATTCTACAAAGCAACTTCAATGGCTTCCGCAAGACTTTCTAAATCTGAACCAAA
CTTACCTTGAGAGAGAAGACGGTGAGTGATCCGAAAGTATCCATGGATTTGGAGTTCTCT
GGGATTTGTGAATGAAGAAGACAGATGAGAGAAACATACATTCCTCTGTTCAACGAATCT
CCAACGAACATCATCTTCTTCCCTCTTAAGCTCTCCAACATCACCGTCGCATTAAACCTA
TTAACACATTCAACAACCACAGAGCAAATCCGATCTGAAGATCAATCACCGAATCGCATT
CAAAATCGAACGGAGAGAGAGACTAACGAAGGAAGTGAGCAGGAATCAGGTCGCCATCTC
CATGACTGATAATCACTATCGGGACGGCCATGCGTGCGGCAAGTGAGCTGCGGCTGAATG
TACGGACACTCGGATTCTCGGTAAAGTGG

Last Updated on 06.12.2018 16:11