Sequences from confirmation amplicon


Confirmation sequences for line SALK_086913c and hit At3g62870 are shown.
Line ID SALK_086913c
Locus At3g62870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23243040
Primers used for PCR 22ih - LBb3, show primer details
   
Sequence Name 62-K114145-SALK_086913C-R210
GenBank Accession LN485798 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114145-SALK_086913C-R210
GTTACACCACAATATATTTGTTATACATGAGAGTATCATTTCAAAACTATTCCATTGTCA
AAAGCTGATTCAGCTAATTACCTTGATGGCCTCGAGGATTTTGCTGAACTCAAGCTTGTC
CTCGTTCTTGACAGTGGTCAAGCACAAGGCAGCAGCAGTCTTCTGGTGAACGACCTGGAG
AGTAGAAGCAGGAAATGAACATAAATTAGGGTGAAAACATTAAAGTAAGAAAAACTCTTA
ATGGAAGTTTACATCCATGGAGTGGTGAATTATTTACCGCTCCAAGACGAGATTTTCCCT
TAACAATGCAGTAAGGGACTTCCATTTTCCTGCAGAGTGCAGGTAACCAGACCACCAACT
CGATAGGGTCAACATCGTGTGCAATGACCACAAGCTGTGCCTTGTTCTGCTCGATGAGGT
AAGTCACATGGTTGAGACCATACTTAAACCAAATGGG
   
Sequence Name 73-K114146-SALK_086913C-22ih
GenBank Accession LN486082 [GenBank]
Sequencing primer gene specific primer, 22ih: 5'-GCCCATTGTTGTTAAGTATGGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114146-SALK_086913C-22ih
TCATTGCACACNATGTTGACCCTATCGAGTTGGTGGTCTGGTTACCTGCACTCTGCAGGA
AAATGGAAGTCCCTTACTGCATTGTTAAGGGAAAATCTCGTCTTGGAGCGGTAAATAATT
CACCACTCCATGGATGTAAACTTCCATTAAGAGTTTTTCTTACTTTAATGTTTTCACCCT
AATTTATGTTCATTTCCTGCTTCTACTCTCCAGGTCGTTCACCAGAAGACTGCTGCTGCC
TTGTGCTTGACCACTGTCAAGAACGAGGACAAGCTTGAGTTCAGCAAAATCCTCGAGGCC
ATCAAGGTAATTAGCTGAATCAGCTTTTGACAATGGAATAGTTTTGAAATGATACTCTCA
TGTATAACAAATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATT
GCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCT
GATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCC
CCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC

Last Updated on 06.12.2018 16:11