Sequences from confirmation amplicon


Confirmation sequences for line SALK_087610 and hit At5g15600 are shown.
Line ID SALK_087610
Locus At5g15600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5078243
Primers used for PCR 11ei - LBb3, show primer details
   
Sequence Name 25-K111273-SALK_087610C-11ei
GenBank Accession LN485001 [GenBank]
Sequencing primer gene specific primer, 11ei: 5'-ATTCAAAACAATCCTCAAAACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111273-SALK_087610C-11ei
AGAGATAGTTAGACATTTTACATTTTTTCAAGGGTCAAATGCATGAGATGTTTGTCGTGG
AAATAGAAATCATTTGTCAGATCCACAAGGACCAGATCCAAACAAGTAACCCAAAGAAGA
TCCTCCACCTGGTGCAGCATGAACCTTCGTAGATGGTCGTTCCTGTTTCTCAAACCACAA
AACCAAATCAGTTTTAAATTCAAGTTCCTCAATCAAACTTGAATTTAAAACTCTTACCGT
GAGGAAGTTGCCACAGTTTTGTCCATCTGATCTGTAGTAATTATTAGGACTTCCTCTCAC
ACCAGCCGAGTTCTCCTCTGTCTTGTTTTTATCTCCGGTTGTTGTAGTTGTGGTCGTGGT
CTTGGGTCTACCTCCAGCTCCATCCGTTGTTGTTGTGGTGGTCGTGGTGGTGGTAAAACC
GGTTTTAGCGTTGGGCTTGTTTGGTTTGGGAACAGACTCGCCGGAACCAAAAAGATAACC
GAGAGAGCTCTCTCCGCCGCCGCTGTTAACTCCTCTAGCTTTCCCCATTTTCCGGCGAGA
ATATTCCGCCGCCGATTCTTGAAATTCTAACAATGTGTAACATTGTAAACATAATCCAGA
TCAATATATCTATAATTAATTTGATTACTTGTAAACCATTAAAGACACATAGGAGAGAGT
TTTTTTTTTTGAACCTTTTTTTTCCGGATTGGACCCTAAAACAATTTAATAACACTTTGC
GAACTTTTTAAAGGTACGGGGGGGGTTTTTCTTTTCACCAGGAAAACGGGCAACAGTTAA
TTGCCCTTCACCGCCGGGCCTTAAA
   
Sequence Name 09-K111272-SALK_087610C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111272-SALK_087610C-R210
AAGGTACCCCTTTGTTAAAATTCCAAAAACCGGCGGCGAAATATCCCCCCCGAAAAAGGG
GAAAACCTAAAGAATTTAACACCGGCGGCGAAAAAACCTCTCCCGGTAATCTTTTGGGTC
CCGGCAAGTCTGTCCCCAAACCAAACAACCCCAACCCTAAACCCGGTTTTCCCCCCCCCC
CAACCCCCACAACACCAACGAAGGGACCTGGAGGTAAACCCAAAACCCCAACCCCAACTC
CAACACCCGGAAATAAAACCAAAACAAAGAAAACCTCGCCGGGGGTGAAAGAAATCCCTA
ATATTTCCTCCGAATCAAAGGGACAAACCTGGGGCACCTCCCCCACGGAAAAATTTTAAA
TTTCAATTTTGATTGAGAACCTGGAATTTAAAACGGATTGGGTTTTGGGGTTGGAAAAAC
GGGACCAACCTTCTCCAAGGGTCCAGGCTGCCCCAGGGGAAGAACCTCCTTGGGGTAACT
TGTTGGAATCGGGCCCTGGGGAATCTAACAAATAATTTCTATTCCCCCAACAAACTTCTA
AGGCATTTAACCCTTTAAAAAAATGTAAAATTTGTCCAAACCTTTATCCTTTACCCTTAC
AACCGGGTTTTAA

Last Updated on 06.12.2018 16:11