Sequences from confirmation amplicon


Confirmation sequences for line SALK_090646c and hit At5g14120 are shown.
Line ID SALK_090646c
Locus At5g14120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4556598
Primers used for PCR 16gq - LBb3, show primer details
   
Sequence Name 01-K112772-SALK_090646C-R210
GenBank Accession LN484281 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112772-SALK_090646C-R210
TATTTGTTTCCCACAATCCATCCGTAACCGATGAGGTTCTGAACGGCGCCAACAAGCCAG
TGAGGCCCAAAGAGGCAAGATCTCAGAGAGAGTACCGGCGATGATTCCGACACTATCACC
GAGATCCTTGGCGACTCCAAGTCTGGAAAGCTCCTTCTGATTATAGTTTAAGGAGCTTTT
GATAACCGGAGAAATGCTTCCGAACAAATAACCGATGCCAGCACAAGACTGTATCCACAT
GGCAGCCACGAAGACGAGCCATCTGTTGTTTATGAAAGATACAAACTTTTCTCGTGTAGT
CGACGCCATTGAGAAAAATGCTTTCTGGGTTTCCTTTGTTTCTTCTCTCCGCCACCGGGA
GAATCCTGGAAGAATCAGATCAAAACACGATTGCGAAATCTGTGGTGTTAGAAAGAGAAA
AGAAATTTTTTTTTTTGAATATTCGGGTCTGTCTATCGGTCTGGTCTTTCACACAATTTT
CTCCTTTTATGGTCTTAAATTGGACAAAAAGGGACAAGACTTAGAAAAGATAAACCCATT
CTGTGAAATATTTACTATTGCAAATATGCGATTCCGGTTTAGTTTGTTAATTACGATAAT
TTTAATATGATCAAAATTTGGTTACAACTTGTAAGTGGATTTTAGTGAGGAAGAAAAAAA
AGAAAAAAGTGGATTTTAGTGAAGAAGTCGTATACAAATTGTAAAACAAAAGGCTCGTTG
ATAATTATGAGTAGTAGTCAGTCCCCGGGGGACTGTTGTGAAT
   
Sequence Name 02-K112773-SALK_090646C-16gq
GenBank Accession LN484308 [GenBank]
Sequencing primer gene specific primer, 16gq: 5'-ACAGTGACGAAATTCACAACAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112773-SALK_090646C-16gq
ACGAGCCTTTTGTTCTCAATTTGTATACGACTTCTTCACTAAAATCCACTTTTTTCTTTT
TCTTCTTCCTCACTAAAATCCACTTACAAGTTGTAACCAAATTTTGATCATATTAAAATT
ATCGTAATTAACAAACTAAACAGGAATCGCATATCTGCAATAGTAACTATTTCACAGATT
GGTTTTATCTTTTCTATGTCTTGTCCTTTTTTGTCCAATTTAAGACCATAAAAGGAGAAA
ATCGTGTGAAAGACAAGACAGATAGACAGACACGAATATTCAAAAAAAAAAATTTCTTTT
CTCTTTCTAACACCACAAATTTCGCAATCGGGTTTTGATCTGATTCTTCCAGGATTCTCC
CGGTGGCGGAGAGAAAAAACAAAGGAAACCCAAAAAGCATTTTTCTCAATGGCGTCAACT
ACACAAAAAAAGTTTGTATCTTTCATAAACAACAAATGGCTCGTCTTCGGGGCTGCCATG
TGGATACAGTCTTGTGCTGGCATCGGTTATTTGTTCGGAAGCATTTCTCCGGTTATCAAA
AGCTCCTTAAACTATAATCAAAAGGAGCTTTCCAAACTTGGAGTCGCCAAGGATCTCGGT
GATAGTGTCGGATTCATCGCCGGTACTCTCTCTAAAATCTTGCCTCTTTGGGCCGCTCTT
CTGGTTGGCGCCGTTCAAAACCTCATCGGTTACGGATGGATTGTGGTGTAAACAAATTGA
CGCTAAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
ACCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCAAAAATCCTGTT

Last Updated on 06.12.2018 16:11