Sequences from confirmation amplicon


Confirmation sequences for line SALK_093125 and hit At5g17540 are shown.
Line ID SALK_093125
Locus At5g17540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5782667
Primers used for PCR 53fm - LBb3, show primer details
   
Sequence Name 88-K111509-SALK_093125C-53fm
GenBank Accession LN486317 [GenBank]
Sequencing primer gene specific primer, 53fm: 5'-GTACAACGATCCCTATCTCACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K111509-SALK_093125C-53fm
TGCCCCCGGAGGTCTTCACTCCGGCTGTACAATTCCTCCGTAAACTGGTTTCCCCCAAAT
TCCGAAATCAATATCTGCGAAAATTCTTACATCCGAAACCAAGTAAGCTCCGTCCGACGA
GAAGCTTGGTCTTCCCTTTATCACCATCAGATCCGCAAGTGATCGCATGTACTCCTCCGT
CACGCTCGATTTCGCCTCTTTTATTAGTCTCAGAGCAAACTCTAACGGTTTCTTAGTTAG
TTCATTAGCTGTTGCGATTGCGACTGGGAACGCAAAGGCATTTCCGTAGTATCCTCGAGG
TAGTGGTGGATTTTTAAGCTTAGAACGTGCGTTGACAATTAATATGAGCCGCATCTCCTT
GTCCTGGTCTGGTCGTAGAGCGATGGTGCGATAACGCCATAAGAAAGACGTTAGCATTTC
CATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 74-K111508-SALK_093125C-R210
GenBank Accession LN486102 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K111508-SALK_093125C-R210
GTCATTTGTTTACCACAATATGGAAATGCTAACGTCTTTCTTATGGCGTTATCGCACCAT
CGCTCTACGACCAGACCAGGACAAGGAGATGCGGCTCATATTAATTGTCAACGCACGTTC
TAAGCTTAAAAATCCACCACTACCTCGAGGATACTACGGAAATGCCTTTGCGTTCCCAGT
CGCAATCGCAACAGCTAATGAACTAACTAAGAAACCGTTAGAGTTTGCTCTGAGACTAAT
AAAAGAGGCGAAATCGAGCGTGACGGAGGAGTACATGCGATCACTTGCGGATCTGATGGT
GATAAAGGGAAGACCAAGCTTCTCGTCGGACGGAGCTTACTTGGTTTCGGATGTAAGAAT
TTTCGCAGATATTGATTTCGGAATTTGGGGGAAACCAGTTTACGGAGGAATTGGTACAGC
CGGAGTTGAAGACCTTCCGGGGGCTAGCTTCTACGTTTCATTTGAGAAGAGAAATGGTGA
GATAGGGATCGTTGTA

Last Updated on 06.12.2018 16:11