Sequences from confirmation amplicon


Confirmation sequences for line SALK_093557c and hit At4g14100 are shown.
Line ID SALK_093557c
Locus At4g14100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8120917
Primers used for PCR 03ii - LBb3, show primer details
   
Sequence Name 37-K112623-SALK_093557C-03ii
GenBank Accession LN485250 [GenBank]
Sequencing primer gene specific primer, 03ii: 5'-ACTAAATCTGCAGAGAGCTCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K112623-SALK_093557C-03ii
CTGACTTTACAAACCGAGAACTCTCCTACTCTCCTAAGATATTTCAATTTCCTACTCAAA
ATGAAAAACTCAAACCTTACACAATGACTATCCTATAAACCAGACACTATAACTAAGGTC
ATTGTTGCTTCAAATTGGAGCAATCTCTGGTTTTCTAACTCTCATTCCCAGTGAATTCAC
ACAAAGCAATTAACAAAGTTAACCAAATCCCAAATTCGAATTTGGTTCCCTATTCTACAG
CCTAACCGTATTCTGAGATCTGTAACAGAGTCATGAACAGAAAATACCAACCTCGAGCTG
ACCGGAGCGGCACGATTTTGACTCGTCGAGCGTGTAAAAGAAGGAAGTACCATTGTTCCA
TTCAAGGTCGTAGGTAATACCACCGAGCTGCTCCTGGATGATATTGAAATTACGACCGTT
GGTCCAGTCAACTTAATAATTTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGA
AAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 13-K112622-SALK_093557C-R210
GenBank Accession LN484641 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112622-SALK_093557C-R210
GTTGACTGGACCAACGGTCGTAATTTCATATCATCCAGGAGCAGCTCGGTGGTATTACCT
ACGACCTTGAATGGAACAATGGTACTTCCTTCTTTTACACGCTCGACGAGTCAAAATCGT
GCCGCTCCGGTCAGCTCGAGGTTGGTATTTTCTGTTCATGACTCTGTTACAGATCTCAGA
ATACGGTTAGGCTGTAGAATAGGGAACCAAATTCGAATTTGGGATTTGGTTAACTTTGTT
AATTGCTTTGTGTGAATTCACTGGGAATGAGAGTTAGAAAACCAGAGATTGCTCCAATTT
GAAGCAACAATGACCTTAGTTATAGTGTCTGGTTTATAGGATAGTCATTGTGTAAGGTTT
GAGTTTTTCATTTTGAGTAGGAAATTGAAATATCTTAGGAGAGTAGGAGAGTTCTCGGTT
TTGTTAAAGTCAGGTGATTTCACAAATCTTCATTTGATGAAAATGGAGCTCTCTGCGNAT
TTAG

Last Updated on 06.12.2018 16:11