Sequences from confirmation amplicon


Confirmation sequences for line SALK_096152 and hit At4g17310 are shown.
Line ID SALK_096152
Locus At4g17310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9686921
Primers used for PCR 05vw - LBb3, show primer details
   
Sequence Name 67-K118240-SALK_096152C-05vw
GenBank Accession LN485941 [GenBank]
Sequencing primer gene specific primer, 05vw: 5'-CCCAAAAGTGAAGCTTTCTAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K118240-SALK_096152C-05vw
CGTGAGCTCGCGAAGCTCCAGAAGCACTAGTATCGAAATAGAAACGACAATACCAGTGGA
TAGTGAACTTTTCCGAGCTGAAAAACTCGTTCAAGAACTTCGAACGAATTACCATTGTAG
AGAACGAATTGAGCTCAAAAACAAAGAAATCAGATCAAAATCTATGGAGATCTGAGCTTG
ATAGCTCTGATACCATATTACGAATGAGAGAACGAGAGAGTTTGTTAGAGAAAGTTTGTT
GAAATGATTTTCTCATCAACTCGATCAGAATAATCTTTACATTGCTATATATATACATTT
GGTACTAAAGCTAATCCACAACTATACATCCGGTTAAGTTAACCAATAATGTAAACCAAA
TATAACCAACTCCAATAAAAGCCCAAAAGGAGCGAAATATTTGTTTGCCTAAAAACCTTA
TAGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAATCGGGCAAAAT
   
Sequence Name 44-K118239-SALK_096152C-R210
GenBank Accession LN485382 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118239-SALK_096152C-R210
GGTTTTAGGCAAACAATATTTCGCTCCTTTTGGGCTTTTATTGGAGTTGGTTATATTTGG
TTTACATTATTGGTTAACTTAACCGGATGTATAGTTGTGGATTAGCTTTAGTACCAAATG
TATATATATAGCAATGTAAAGATTATTCTGATCGAGTTGATGAGAAAATCATTTCAACAA
ACTTTCTCTAACAAACTCTCTCGTTCTCTCATTCGTAATATGGTATCAGAGCTATCAAGC
TCAGATCTCCATAGATTTTGATCTGATTTCTTTGTTTTTGAGCTCAATTCGTTCTCTACA
ATGGTAACTCGTTCGAAGTCTTGAACGAGTTTTTCAGCTCGGAAAAGTTCACTATCCACT
GGTATTGTCGTTTCTATTTCGATCTCGTGCTTCTGGAGCTTCGCGAGCTCACGATTCACC
GGATATTTCAAATCCGAACTAGCTTTTAATGGCATTTATAGGATTAGAAAGCTTCACTTT
TGGG

Last Updated on 06.12.2018 16:11