Sequences from confirmation amplicon


Confirmation sequences for line SALK_097816 and hit At5g27850 are shown.
Line ID SALK_097816
Locus At5g27850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9873856
Primers used for PCR 29lu - LBb3, show primer details
   
Sequence Name 87-K114279-SALK_097816C-29lu
GenBank Accession LN486308 [GenBank]
Sequencing primer gene specific primer, 29lu: 5'-GCTCCTAAGTCCGATGATGTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K114279-SALK_097816C-29lu
TTTTACTTATGTTTTCTCTGTCTGTACATTTTGGTTTATTGAGATTTGTTTGTGTTTTTG
ATAAATTTTGTAGCTTTACCGGTTTTTGGTGAGGAGATCTAACAGCAACTTCAATGCTGT
GATTCTGAAAAGGCTTTTCATGAGCAAAGTCAACAAAGCTCCTCTTTCACTCTCTAGGCT
TGTAGAGTTCATGACTGGCAAGGTTCGATCTTTTTGAGACCCATCTCTCTTTATATACAT
CTTGAGTCTGTCCTTGGTCTTAATTTGGAACCCCATTTATTTTATTTTCATTCTGTGAAG
TTATGAGTTTTGACGTTGTCCTGTTATGTTAGTATGTGAGAACAAATGTACAAACTTGAT
TTTGTATCTTACTATTCAGTGATGGTTAGAGTACTCAAATGTTGTGGTTTAAACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
GA
   
Sequence Name 73-K114278-SALK_097816C-R210
GenBank Accession LN486083 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K114278-SALK_097816C-R210
CATTTGTTTAACCACAACATTTGAGTACTCTAACCATCACTGAATAGTAAGATACAAAAT
CAAGTTTGTACATTTGTTCTCACATACTAACATAACAGGACAACGTCAAAACTCATAACT
TCACAGAATGAAAATAAAATAAATGGGGTTCCAAATTAAGACCAAGGACAGACTCAAGAT
GTATATAAAGAGAGATGGGTCTCAAAAAGATCGAACCTTGCCAGTCATGAACTCTACAAG
CCTAGAGAGTGAAAGAGGAGCTTTGTTGACTTTGCTCATGAAAAGCCTTTTCAGAATCAC
AGCATTGAAGTTGCTGTTAGATCTCCTCACCAAAAACCGGTAAAGCTACAAAATTTATCA
AAAACACAAACAAATCTCAATAAACCAAAATGTAACAGACAGAGAAACATAAGTAAAAGA
CATGTCAATATTTGTTACCTTGACGAGAAGCTTTAGGTAGACATCATC

Last Updated on 06.12.2018 16:11