Sequences from confirmation amplicon


Confirmation sequences for line SALK_101916 and hit At2g26190 are shown.
Line ID SALK_101916
Locus At2g26190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11148854
Primers used for PCR 54vi - LBb3, show primer details
   
Sequence Name 59-K117945-SALK_101916C-54vi
GenBank Accession LN485725 [GenBank]
Sequencing primer gene specific primer, 54vi: 5'-TTCATTGAAGTTGTCTTCTGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117945-SALK_101916C-54vi
AATATGTGTTTTGATTAGATCCTCGACATAAAAGCTAAGAGAACCAAACTAGAAAGCAAA
ACTGAATCGGAACACACCTCGAGGATCCCCTCGCGAGCAACCAATCTTCCTGCAGCAGTT
GTGGCACCTCCAGACAAGAAACTCGAATGTTGGAAACGACCTTTCTTCTTCTGCCCTACG
TATAAGGTTCTAGTTGTGCTAAGTACAAAAATTGACTTGGAATCCTCTGTTGAATTGATC
AGAGTCATGCTTTGTTTATTCATTAGCTTTCCATCTTCTACTATCACTTCGTATGCTTCT
CTCTCCAGCTGCAGGTTGAATAACACAAACGTGTATGAGCAAAAAGAAGATAAGTACCCA
TTGCGGACGCTTATAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTT
TTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG
AAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAAT
   
Sequence Name 24-K117944-SALK_101916C-R210
GenBank Accession LN484986 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K117944-SALK_101916C-R210
GTCATTTGTTATAGCGTCCGCAATGGGTACTTATCTTCTTTTTGCTCATACACGTTTGTG
TTATTCAACCTGCAGCTGGAGAGAGAAGCATACGAAGTGATAGTAGAAGATGGAAAGCTA
ATGAATAAACAAAGCATGACTCTGATCAATTCAACAGAGGATTCCAAGTCAATTTTTGTA
CTTAGCACAACTAGAACCTTATACGTAGGGCAGAAGAAGAAAGGTCGTTTCCAACATTCG
AGTTTCTTGTCTGGAGGTGCCACAACTGCTGCAGGAAGATTGGTTGCTCGCGAGGGGATC
CTCGAGGTGTGTTCCGATTCAGTTTTGCTTTCTAGTTTGGTTCTCTTAGCTTTTATGTCG
AGGATCTAATCAAAACACATATTTTCTCAGGCTATATGGCCATACAGTGGACACTACCTC
CCAACAGAAGACAACTTCAATGA

Last Updated on 06.12.2018 16:11