Sequences from confirmation amplicon


Confirmation sequences for line SALK_102395c and hit At5g55660 are shown.
Line ID SALK_102395c
Locus At5g55660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22541619
Primers used for PCR 46ew - LBb3, show primer details
   
Sequence Name 17-K111285-SALK_102395C-46ew
GenBank Accession LN484745 [GenBank]
Sequencing primer gene specific primer, 46ew: 5'-CTCTGTAAAGAACCCAAAGAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K111285-SALK_102395C-46ew
AAGACGAATTTCTAGCACAAGGATTTAAACCTCCAGTTCTAAGCTATTGAGTAATACCTT
TGCTGATCGTTTTGAAGATGAACTTCCAGCTGCAGGTGAACTTTTCTTAGGAGTTCTTTT
GCGCTTCACTCCCTATTCAATAGAAAAAATAGAAAGTTGTAATATGCACAATAAATTTTC
AGGCTTAATTGCTGAACAGAAGGCCTTTCCAGCACCAAACTTACCTTCTCTTTCTCATTG
ACTAGAACATCAGTTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGC
GGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA
TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
AGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATANGCAAAA
   
Sequence Name 06-K111284-SALK_102395C-R210
GenBank Accession LN484427 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K111284-SALK_102395C-R210
CATTTGTTTACCCACAACTGATGTTCTAGTCAATGAGAAAGAGAAGGTAAGTTTGGTGCT
GGAAAGGCCTTCTGTTCAGCAATTAAGCCTGAAAATTTATTGTGCATATTACAACTTTCT
ATTTTTTCTATTGAATAGGGAGTGAAGCGCAAAAGAACTCCTAAGAAAAGTTCACCTGCA
GCTGGAAGTTCATCTTCAAAACGATCAGCAAAGGTATTACTCAATAGCTTAGAACTGGAG
GTTTAAATCCTTGTGCTAGAAATTTCGTCTTTGTGTCTGGTCCAAGTACCTGAATTCTGA
TTCGTTTCTTTGGGTTCTTTACAGAG

Last Updated on 06.12.2018 16:11