Sequences from confirmation amplicon


Confirmation sequences for line SALK_103154c and hit At1g76760 are shown.
Line ID SALK_103154c
Locus At1g76760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28812721
Primers used for PCR 28ht - LBb3, show primer details
   
Sequence Name 83-K112669-SALK_103154C-28ht
GenBank Accession LN486258 [GenBank]
Sequencing primer gene specific primer, 28ht: 5'-TCATATGATTGAGCCTGAAGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K112669-SALK_103154C-28ht
TTTGGGACGGGCCTATACATGTGGGCCTAGAAATGTTTGTGGGCCTATAACAAAAAAGAT
ACGGTGTACTTGACCGTAGTGGATCATCATTGTCTTTCTAGTACCTTGTGTCATTTTCTT
TCAGAGTTCGTTCTCTCTTTTTGGTCAATTCGAGTTTTTTTTCCACAAGTGTCCAACTTT
TTTTGTCATCATCAACCTAACCAACCGCCAACGCCGAATCAAGAGATCGTTTGTTTTTTT
CTGATTCTTTCATGGCGTCAATTTCTCTCTCTTCTTCTACGGTTCCGTCGCTGAATTCGA
AAGAATCGTCAGGTGTTTCTGCTTTCGCTTCTCGTTCTATCTCGGCCGTCAAATTTCAGT
TTCCGGTTCGTCGGGTTCGAACCGGAGACTTAAAGTTTCCATCTTTATCTTCAACGACTC
GATGCACTCCTCGACGTGTAATCGCCTTTCTCTGCTTAAACATCTTTTTCCAAAGCTTAC
TAACAAATAATGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGAC
GTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGC
CCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCA
GGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 73-K112668-SALK_103154C-R210
GenBank Accession LN486077 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K112668-SALK_103154C-R210
CGTCATTTGTTTACCCCATTATTTGTTAGTAAGCTTTGGAAAAAGATGTTTAAGCAGAGA
AAGGCGATTACACGTCGAGGAGTGCATCGAGTCGTTGAAGATAAAGATGGAAACTTTAAG
TCTCCGGTTCGAACCCGACGAACCGGAAACTGAAATTTGACGGCCGAGATAGAACGAGAA
GCGAAAGCAGAAACACCTGACGATTCTTTCGAATTCAGCGACGGAACCGTAGAAGAAGAG
AGAGAAATTGACGCCATGAAAGAATCAGAAAAAAACAAACGATCTCTTGATTCGGCGTTG
GCGGTTGGTTAGGTTGATGATGACAAAAAAAGTTGGACACTTGTGGAAAAAAAACTCGAA
TTGACCAAAAAGAGAGAACGAACTCTGAAAGAAAATGACACAAGGTACTAGAAAGACAAT
GATGATCCACTACGGTCAAGTACACCGTATCTTTTTTGTTATAGGCCCACAAACATGTTC
TAGGCCCACATGTTATAGGCCCGTTCCCAAACACCGATCACTCTTCAATTTAACTTTTTG
CTCACAATCTTCAGGCTCAATCATATGA

Last Updated on 06.12.2018 16:11