Sequences from confirmation amplicon


Confirmation sequences for line SALK_106202c and hit At3g53840 are shown.
Line ID SALK_106202c
Locus At3g53840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19946925
Primers used for PCR 27ie - LBb3, show primer details
   
Sequence Name 19-K113191-SALK_106202C-27ie
GenBank Accession LN484818 [GenBank]
Sequencing primer gene specific primer, 27ie: 5'-CAGATATGTGTTCGGCTTATCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K113191-SALK_106202C-27ie
GGTTACAATGGGGCGAACAGCCGTGGAAATCTATGGGAAGCTCCTAGAGAACCGGTTTGT
AAGAGTCAAGGAGATTGCAGAGATTTATTGAATTCAGTTTGTTCGAACGATTCAACGAAT
TTAGGACAGAAGAGATGTTTCTGCAAGAAAGGGTTTCAATGGGACTCGGTCAACGCAGTA
TGTGAAGGTCCGGTCCGGTTTTGTGATATGGTTTTTGATTATTTGAGATTTGAGTCTTTT
ACTAAAAGCAAAATTGTTATTTGTTTGTTTAGTTAACAGGTGCTCTAAGCGTAAAAGTTG
CAAGCGTTGGAGCAATTTACCTTTATTAGGAGGTAATCAAAATTAAATCAGATGGTTGAA
CCGGGTTAGATTCGGTTACATTCTTTGGTTTGGGTATCTGATTTCCAATTTTTCTATACC
GAGAAGGAAACCAAACATTCTTTGGTTTGGACTGATTAGTTTCTGTTATTGAACAGGTTT
AGCCGGTGGTGTGGGAGCAATATTGATCGCCGGATTCATAACGAAGACGATAGTCTCGAA
GCAGAATCGGAGAATCGCCGGAAACCAATCATGGGCAAGCGTAAGGAAACTTCATCGAAA
CCTGTTGAGTATCAACAGTACCGGACTAGATAGAATCTTCACCGGCAAAGAAATCGTCAA
AGCCACAGATAATTTCGCCAAATCAAATCTCCTAGGCTTCGGTGGATTCGGCGAAGTTTT
CAAAGGAAATCTCGATGACGGAACCACCGTAGCTGTGAAACGCGCCAAACTCGGAAACGA
AAAGAGCATTTACCCAATCGTCAACGAGGTTCAGATTCTCTGTCATAAGCCCAGACAACT
TAATAACACATTGC
   
Sequence Name 08-K113190-SALK_106202C-R210
GenBank Accession LN484493 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K113190-SALK_106202C-R210
GCGCGCTTCACAGCTCCCTTAGAAACGTCATCGAGATTTCCTTTGAAAACTTCGCCGAAT
CCACCGAAGCCTAGGAGATTTGATTTGGCGAAATTCTCTGTGGCTTTGACGATTTCTTTG
CCGGTGAAGATTCTATCTAGTCCGGTACTGTTGATACTCAACAGGTTTCGATGAAGTTTC
CTTACGCTTGCCCATGATTGGTTTCCGGCGATTCTCCGATTCTGCTTCGAGACTATCGTC
TTCGTTATGAATCCGGCGATCAATATTGCTCCCACACCACCGGCTAAACCTGTTCAATAA
CAGAAACTAATCAGTCCAAACCAAAGAATGTTTGGTTTCCTTCTCGGTATAGAAAAATTG
GAAATCAGATACCCAAACCAAAGAATGTAACCGAATCTAACCCGGTTCAACCATCTGATT
TAATTTTGATTACCTCCTAATAAAGGTAAATTGCTCCAACGCTTGCAACTTTTACGCTTA
GAGCGCCTGTTAACTAAACAAACAAATAACAATTTTGCTTTTAGTAAAAGACTCAAATCT
CAAATAATCAAAAACCATATCA

Last Updated on 06.12.2018 16:11