Sequences from confirmation amplicon


Confirmation sequences for line SALK_108611c and hit At3g63000 are shown.
Line ID SALK_108611c
Locus At3g63000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23284324
Primers used for PCR 20gq - LBb3, show primer details
   
Sequence Name 10-K114130-SALK_108611C-R210
GenBank Accession LN484555 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114130-SALK_108611C-R210
TTTTTGTCAGCATTGTCATTGCGTCTCTGAGGATGGTCAGGTTGAGGTGAATTTCATCTA
CGAGCCACCGCAGCAAGGGATGGAGGATAATCTGATTCTGATGAGAGACTCTGAGGAAGA
GAAACGTGTTGATGCTATTGCTTTAGGGTTAGGGATGAGGAGAGTTGGGTTTATATTCAA
CCAGACTGTGACTCAGGACAAGAAGGAGTACACTTTGTCTAACGTTGAGGTGTTGCTTGC
GGCTCAGCTTCACGCTGAGAGCGAATTGAAGGAGTGGGTCACTGCTGTTGTGAAGCTTGA
GATCAATGAGGATGGTGGTGCTGATGTTCACTTTGAGCCGTTTCAGATGAGTGATATGTG
CGTTAGGTTGTTTAAGGAAGGATGGTTCGAGACTGAGATTGGACCTGAGGATGATCCCAA
GCTCTCCAAGTTGAAGAAAGAGGTTGTTGTTGGAGTCAAGGATGTTAAAGAAGTCGATAA
TGATTTCTTCCTTGTTCTCGTCAAGATCTTGGATCATCAGGTTCGTTTTTGTACCCTTTT
AGCTTATGGAACCAT
   
Sequence Name 20-K114131-SALK_108611C-20gq
GenBank Accession LN484854 [GenBank]
Sequencing primer gene specific primer, 20gq: 5'-AATGGTTCCATAAGCTAAAAGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K114131-SALK_108611C-20gq
GAGACAGATCTTGACAGACAGGAAGAATCATTATCGACTTCTTTACATCCTTGACTCCAC
AACAACCTCTTTCTTCAACTTGGAGAGCTTGGGATCATCCTCAGGTCCAATCTCAGTCTC
GAACCATCCTTCCTTAAACAACCTAACGCACATATCACTCATCTGAAACGGCTCAAAGTG
AACATCAGCACCACCATCCTCATTGATCTCAAGCTTCACAACAGCAGTGACCCACTCCTT
CAATTCGCTCTCAGCGTGAAGCTGAGCCGCAAGCAACACCTCAACGTTAGACAAAGTGTA
CTCCTTCTTGTCCTGAGTCACAGTCTGGTTGAATATAAACCCAACTCTCCTCATCCCTAA
CCCTAAAGCAATAGCATCAACACGTTTCTCTTCCTCAGAGTCTCTCATCAGAATCAGATT
ATCCTCCATCCCTTGCTGCGGTGGCTCGTAGATGAAATTCACCTCAACCTGACCATCCTC
AGAGACGCAATGACAATGCTGACAAAAACTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTC

Last Updated on 06.12.2018 16:11