Sequences from confirmation amplicon


Confirmation sequences for line SALK_114435 and hit At1g47470 are shown.
Line ID SALK_114435
Locus At1g47470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17415246
Primers used for PCR CG01 - R204, show primer details
   
Sequence Name 55-K107203-SALK_114435C-CG01
GenBank Accession LN485617 [GenBank]
Sequencing primer gene specific primer, CG01: 5'-GAAAATTCATATGATCCGTGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107203-SALK_114435C-CG01
TAAAATATTTAGAGTATAATACAAAATTAGAAATAATATTTTGAAATTCAACTTTGAAAG
TCTATATATATAAATTAAACTTCATATCCGATTAGATCGAAAATGAATATTGAATTCGGA
ACATTTTACCACCCCAGCGAAATCATGTTATATTTGAAAGTTTTCAGTGTGCATATGCGT
TTTTAGTTTATATTGGATGGATTAATTTGAATGATGAATAAAAACGTAAAATATTGTAAG
GGTTTGGCTTCTTGATTACCAAAAATAAAATGGTAGCAGTTGTTTTATAATGGTAATAGT
CAAATTACAAAAACCCGCAGACACATAACACACATAAATATTAGTTTACTGTTCAAAAGA
GACCGGAGCACCAATCTTGCTCCCTACTCTAAAGACACATTCGTTCCATGTTTGTTTGCT
CTTTGGAATGCCTTTAGATGCTTTATTTTTATACTCATATGTGGCGAATATGAGTTTAAC
CAATCCGAGATGGCAATCTTTTCCGATCTTCAATATATCACGACAACATTCATCTGTAAT
TGGCACTGTCTCATCTAACATGTTCTTGAAAACCTCATTTCCGCATTTTGAGCTTGGCTT
TTCAGAGCAAGCCTCAAGAAAATCGAGCTGTTTATGCGAGTAATGGTGAGCAAGCTCCCT
ATCATATTCCATTGCGGGTGAATCAGCTTCTTCGAATGGAGCTTCACCAGGAGCTTCGAC
GTANATATCAAACTTTGAAGATGGAGATATGGCCACATCTTTTGGCACCTCCTGTGAATA
TGGATCAATTTCTTGGGAGAAAATTGGACGAGCCATGTAAAACATTACAATAGACACGAG
CGTTACCATGAGTAGAGATTTTGTCATTTTTTTTATGGATGTAAGAATACTTTTAGTATT
GAATGTAAGATTGATGACNTGAAAACAGAAAACAAGAGATTTTATAGGTTAATCACTAAA
AGATATTTGTTCATAAATTAACGCATT
   
Sequence Name 47-K107202-SALK_114435C-R210
GenBank Accession LN485429 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107202-SALK_114435C-R210
CGTCATTTGTTTACCCACAATGCGTTAATTAATGAACAAATATCTTTTAGTGATTAACCT
ATAAAATCTCTTGTTTTCTTGTTTCATGTCATCAATCTTACATTCAATACTAAAAGTATT
CTTACATCCATAAAAAAAATGACAAAATCTCTACTCATGGTAACGCTCGTGTCTATTGTA
ATGTTTTACATGGCTCGTCCAATTTTCTCCCAAGAAATTGATCCATATTCACAGGAGGTG
CCAAAAGATGTGGCCATATCTCCATCTTCAAAGTTTGATATTTACGTCGAAGCTCCTGGT
GAAGCTCCATTCGAAGAAGCTGATTCACCCGCAATGGAATATGATAGGGAGCTTGCTCAC
CATTACTCGCATAAACAGCTCGATTTTCTTGAGGCTTGCTCTGAAAAGCCAAGCTCAAAA
TGCGGAAATGAGGTTTTCAAGAACATGTTAGATGAGACAGTGCCAATTACAGATGAATGT
TGTCGTGATATATTGAAGATCGGAAAAGATTGCCATCTCGGATTGGTTAAACTCATATTC
GCCACATATGAGTATAAAAATAAAGCATCTAAAGGCATTCCAAAGAGCAAACAAACATGG
AACGAATGTGTCTTTAGAGTAGGGAGCAAGATTGGTGCTCCGGTCTCTTTTGAACAGTAA
ACTAATATTTATGTGTGTTATGTGTCTGCGGGTTTTTGTAATTTGACTATTACCATTATA
AAACAACTGCTACCATTTTATTTTTGGTAATCAAGAAGCCAAACCCTTACAATATTTTAC
GTTTTTATTCATCATTCAAATTAATCCATCCAATATAAACTAAAAACGCATATGCACACT
GAAAACTTTCAAATATAACATGATTTCGCTGGGGTGGTAAAATGTTCCGAATTCAATATT
CATTTTCGATCTAATCGGATATGAAGTTTAATTTATATATATAGACTTTCAAAGTTGAAT
TTCAAA

Last Updated on 06.12.2018 16:11