Sequences from confirmation amplicon


Confirmation sequences for line SALK_124412c and hit At5g41730 are shown.
Line ID SALK_124412c
Locus At5g41730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16686646
Primers used for PCR 37mv - LBb3, show primer details
   
Sequence Name 29-K114624-SALK_124412C-37mv
GenBank Accession LN485110 [GenBank]
Sequencing primer gene specific primer, 37mv: 5'-TGTTCTTTGGTCTCATTTTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114624-SALK_124412C-37mv
TCTCAGTCTTGTGGACATTATTTGCGTTGGTTTTATTGAAAGAAGAGATTTAAGGATTTT
TTAAACACTGATGGAGCAATTCAGACAAATCGGAGAAGTTCTTGGAAGTCTAAATGCACT
AATGGTATTACAAGATGATATCTTGATCAACCAAAGACAATGTTGTTTGTTGTTAGAACT
CTTCAGCTTAGCTTTCAACACGGTCGCAGAAGAGATCAGACAGAATCTAAAGCTTGAAGA
GAAGCATACTAAATGGAGAGCTCTTGAACAACCTTTAAGGGAGCTTTATAGAGTTTTTAA
AGAAGGAGAGTTATATGTTAAGCATTGTATGGATAATAGTGATTGGTGGGGTAAAGTAAT
CAATCTTCATCAGAACAAAGATTGTGTTGAGTTTCATATACACAACTTGTTCTGTTATTT
CTCTGCGGTTGTTGAAGCCATCGAGGCTGCTGGTGAGATTTCGGGTCTTGACCCTTCTGA
GATGGAGAGAAGGAGAGTTGTGTTTTCAAGAAAGTATGATAGAGAGTGGAATGATCCTAA
GATGTTTCAATGGAGGTTTGGGAAACAGTACATACTGACAAATTGACGCTTAGACAACTT
AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGAC
GGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCAC
GCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 13-K114623-SALK_124412C-R210
GenBank Accession LN484646 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114623-SALK_124412C-R210
TACTGTTTCCCAAACCTCCATTGAAACATCTTAGGATCATTCCACTCTCTATCATACTTT
CTTGAAAACACAACTCTCCTTCTCTCCATCTCAGAAGGGTCAAGACCCGAAATCTCACCA
GCAGCCTCGATGGCTTCAACAACCGCAGAGAAATAACAGAACAAGTTGTGTATATGAAAC
TCAACACAATCTTTGTTCTGATGAAGATTGATTACTTTACCCCACCAATCACTATTATCC
ATACAATGCTTAACATATAACTCTCCTTCTTTAAAAACTCTATAAAGCTCCCTTAAAGGT
TGTTCAAGAGCTCTCCATTTAGTATGCTTCTCTTCAAGCTTTAGATTCTGTCTGATCTCT
TCTGCGACCGTGTTGAAAGCTAAGCTGAAGAGTTCTAACAACAAACAACATTGTCTTTGG
TTGATCAAGATATCATCTTGTAATACCATTAGTGCATTTAGACTTCCAAGAACTTCTCCG
ATTTGTCTGAATTGCTCCATCAGTGTTTAAAAAATCCTTAAATCTCTTTCTTTCAATAAA
ACCAACGCAAATAATTGTTCCACAAGACTTGAGATTTATGAATGCTGGAAAACAAAAACA
ATCAAAATGA

Last Updated on 06.12.2018 16:11