Sequences from confirmation amplicon


Confirmation sequences for line SALK_125666c and hit At4g14723 are shown.
Line ID SALK_125666c
Locus At4g14723 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8440716
Primers used for PCR 33uf - LBb3, show primer details
   
Sequence Name 89-K117830-SALK_125666C-33uf
GenBank Accession LN486328 [GenBank]
Sequencing primer gene specific primer, 33uf: 5'-ACCACAACACTTGTCTCTCTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K117830-SALK_125666C-33uf
GGCACCTTCCGTCGCCGCCGTCGCTTTCTTTTAGCTGCACTCGTCACATTTGCTCTCCTC
CACCTCTTCTCCGCCTCCTCCATAGTCTCCGCCGGTAAGAAACCAATAACAATGTGATTT
TTTTTTTCTTGCAACTCAAACCCGAAAATTTCTTGATTTTCTTGATTTCATAATAAAATT
TTTGGTTTGAGTAATTAAAAACGCAATATTTTTTGTAGTCCAATGGTTAAATGTACTTGA
TGTGTAAATGGTGAGCGAGTTAACTCAGTAGTGGTTAAATTCATTTACCAAATGGTAAAT
GGATTGGTCAAAAAACCGGGTCGGATTTACCGGGTGGGTTCATAAGGAGTAATAAGCGGT
TTGGTGGTCCGGGTTCATCACCACCGACGTGTAAATCGAAGTGTGGGAAATGTCACCCGT
GTAAACCGGTTCACGTACCAATTCAACCCGGTTTAAGTATGCCATTGGAGTATTACCCTG
AAGCTTGGCGGTGCAAGTGTGGCAACAAGCTCTTCATGCCCTAAAGTTTGATCAATTATT
AAACAATTCACATTGACCCTAAAACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCC
   
Sequence Name 72-K117829-SALK_125666C-R210
GenBank Accession LN486065 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K117829-SALK_125666C-R210
GATTGTTTATAATTGATCAAACTTTAGGGCATGAAGAGCTTGTTGCCACACTTGCACCGC
CAAGCTTCAGGGTAATACTCCAATGGCATACTTAAACCGGGTTGAATTGGTACGTGAACC
GGTTTACACGGCTGACATTTCCCACACTTCGATCTACACGTCGGTGGTGATGAACCCGGA
CCACCAAACCGCTTATTACTCCTTATGAACCCACCCGGTAAATCCGACCCGGTTCTCTGA
CCAATCCATCTACCATCTGGTAAATGAATTTAACCACTACTGAGTTAACTCGCTCACCAT
TTACACATCAAGTACATTTAACCATTGGACTACAAAAAATATTGCGTTTTTAATTACTCA
AACCAAAAATTTTATTATGAAATCAAGAAAATCAAGAAATTTTCGGGTTTGAGTTGCAAG
AAAAAAAAAATCACATTGTTATTGGTTTCTTACCGGCGGAAACTATGGAGGAGGCGGAAA
AAAGGTGGAGGAAAGCAAATGTGACGAGTGCAGCTAAAAAAAAGCGACGGCGGCAACGGA
AGGTGCCCATTAGTCAAGAACCGGAAAGGATGCGTATGTTAGCAAAGAG

Last Updated on 06.12.2018 16:11