Sequences from confirmation amplicon


Confirmation sequences for line SALK_128264c and hit At5g20500 are shown.
Line ID SALK_128264c
Locus At5g20500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6938948
Primers used for PCR 37lm - LBb3, show primer details
   
Sequence Name 46-K114227-SALK_128264C-37lm
GenBank Accession LN485415 [GenBank]
Sequencing primer gene specific primer, 37lm: 5'-TTTTGTTGAAGGAAAAGGAGTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114227-SALK_128264C-37lm
TCGTTATGAAGACTTGCGGTACTGTTCGCCTTCCAACAATCTCTCCAAGTGCAGTCTGGA
TGCTCCAACCATCTTCTGCAAGACCAAATTTTTACCAACCATTAGTCACAAATGAATCTT
CTACATAGTGATCGGACAAAGTTTCAAATGACAGAAATGTCTGAGAGCTAAGAAACATTG
TTGGATGATGAAGACATGATTCCCTTTAACCTTAAAACACACCTCTTTCATCAAGCTCGA
CAACATAAGGAACTTGATCCAGCTCTCTGAACACTGATTTAGCTTTCTTGCAGTAGCTGT
TGACCAAACCACAAGGAAGCAAAGGAAAAGTAGAGATGAGTCATATACATAGGTACATGA
TCTCTCTACACAACTCAATTAAGGCCAAAGGAAAAGCAAAGAGAACAAATACTGAGAACT
GAACCTGAATATATCTTAAACGAAAATCCATATTGATTTCAACCACCATTTGTAAACAGA
GATAACAAAGTTGTCTCATAAAAATCCAAATCTAACGAATACAAGTTCATTGGGTTCAAG
TTTTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTG
GTTCC
   
Sequence Name 42-K114226-SALK_128264C-R210
GenBank Accession LN485333 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114226-SALK_128264C-R210
ACTTGTATTCGTTAGATTTGGATTTTTATGAGACAACTTTGTTATCTCTGTTTACAAATG
GTGGTTGAAATCAATATGGATTTTCGTTTAAGATATATTCAGGTTCAGTTCTCAGTATTT
GTTCTCTTTGCTTTTCCTTTGGCCTTAATTGAGTTGTGTAGAGAGATCATGTACCTATGT
ATATGACTCATCTCTACTTTTCCTTTGCTTCCTTGTGGTTTGGTCAACAGCTACTGCAAG
AAAGCTAAATCAGTGTTCAGAGAGCTGGATCAAGTTCCTTATGTTGTCGAGCTTGATGAA
AGAGGTGTGTTTTAAGGTTAAAGGGAATCATGTCTTCATCATCCAACAATGTTTCTTAGC
TCTCAGACATTTCTGTCATTTGAAACTTTGTCCGATCACTATGTAGAAGATTCATTTGTG
ACTAATGGTTGGTAAAAATTTGGTCTTGCAGAAGATGGTTGGAGCATCCAGACTGCACTT
GGAGAGATTGTTGGAAGGCGAACAGTACCGCAAGTCTTCATTAACGGAAAACATCTCGGA
GGATCAGATGGTATACTTAACACTCCTTTTCCTTCCACAAA

Last Updated on 06.12.2018 16:11