Sequences from confirmation amplicon


Confirmation sequences for line SALK_128432 and hit At3g58670 are shown.
Line ID SALK_128432
Locus At3g58670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21705268
Primers used for PCR 64fr - LBb3, show primer details
   
Sequence Name 56-K111957-SALK_128432C-64fr
GenBank Accession LN485644 [GenBank]
Sequencing primer gene specific primer, 64fr: 5'-TCAATGAATTGAGAGAATTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K111957-SALK_128432C-64fr
CATTCGATTATGATTCAGTTTTGCAAATAACTGACCTAAATTCTATTCCTGGCAATACTT
TTGCTTCATTGAGTGAGCCCATTTAGGTAGCTGCAGTGATTTTGAATTCGATATGTAAGA
AAAAAGATTAATCATTCACTTGTTAGAGTTCGTCAAAAACACTACTTTGTGAGAGACTCA
GAGCTCCTGGATTTGTAATATTTGTTTTCAGATGTGTTTGTTTAGATTATGGAGCTCTAT
ATGAAGTTAAAGTTGTTTTCTTTTCTCTCCTTTCAGGGCAGTGCTTGAGAATCATTGAAG
AAGGTGATCGCTGGTGCTGTTTTGATAACTCAAAGCCGTTTTGCTGAAAGAGGGTTACCA
AAGTTAGAAGCTCAAGAACTTGGAGCTGTGTCTTCTTTTTGTATCATGCCATATTTTATT
CAGAGGCTTTTCAATACTTGCAAGTCATCTCTTATAGGATTTGTTTGCAGGATATATTGT
GGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTG
TTTGATGGTGGTTCCG
   
Sequence Name 52-K111956-SALK_128432C-R210
GenBank Accession LN485560 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K111956-SALK_128432C-R210
GTCATTTGTTACACACAATATATCCTGCAACAAATCCTATAAGAGATGACTTGCAAGTAT
TGAAAAGCCTCTGAATAAAATATGGCATGATACAAAAAGAAGACACAGCTCCAAGTTCTT
GAGCTTCTAACTTTGGTAACCCTCTTTCAGCAAAACGGCTTTGAGTTATCAAAACAGCAC
CAGCGATCACCTTCTTCAATGATTCTCAAGCACTGCCCTGAAAGGAGAGAAAAGAAAACA
ACTTTAACTTCATATAGAGCTCCATAATCTAAACAAACACATCTGAAAACAAATATTACA
AATCCAGGAGCTCTGAGTCTCTCACAAAGTAGTGTTTTTGACGAACTCTAACAAGTGAAT
GATTAATCTTTTTTCTTACATATCGAATTCAAAATCACTGCAGCTACCTAAATGGGCTCA
CTCAATGAAGCAAAAGTATTGCCAGGAATAGAATTTAGGTCAGTTATTTTGCAAAACTGA
ATCATATATCGAATTGAATTGAATTGAATTGAGCTGTGCCCAGCAAATTCTCTC

Last Updated on 06.12.2018 16:11