Sequences from confirmation amplicon


Confirmation sequences for line SALK_133064c and hit At1g66020 are shown.
Line ID SALK_133064c
Locus At1g66020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24579792
Primers used for PCR CG21 - LBb3, show primer details
   
Sequence Name 64-K107792-SALK_133064C-CG21
GenBank Accession LN485839 [GenBank]
Sequencing primer gene specific primer, CG21: 5'-GTAGTGGTGGTCATGATGAAAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K107792-SALK_133064C-CG21
GGATAACACCAGATTATTTAAAATCGTGTTGAATTTTATATTGAAAACTTTCGAAGTGTT
TCAAAAAGAACTTGAGCCAGAAGGAAGATCTTACACTGTGAAAGCCACTATAGAAGAGGT
AACCAATCAAACTCTTCTCTGTAATTATGATTAATCAATTGAACTCGTTGGACTAATATT
TTGTAGAAGAGCTTATCAAAAGTATATATTTTTTGTAAAGAAGAAACTTATGGTATAGAA
AAACGATTTTGAAAAATAAAAAGTTTGTACACGTAATATGGTTCTATACTTTTGAGTATG
CAGTTTAAGACAGTTACCAAAGGCAACTTTGATCTTGCCAAATGGGCACACGCTGTCCAT
GTGCCTAGCTTTGAGGAGTACATGGAAGTTGGTGAGGAGGAGATCTCAGTGTGTTCGACT
TTGGCAGGTATATTCATGTGTATGGAACAGAAGGCTACAAAAGAAGATTATGAATGGCTA
AAGTCCAGACCTAAATTCATCCAAACTTTATGTGCCAGATGTCGTCTTAAGAATGATATA
ACTGGTTTTGAGGTACAACTATTAAATCATTTCTTCACATCTGGGGTATGTTTCTACTTT
CTTGTTCTATCTAACGTACATATTTATTATACATTTATTGGCTAGGATGACATGAGTAGA
GGATATGTCACGAATGCAGTAAATTGTTATATGAAGCAATATGGAGTTACTAAACAAGAA
GCTTTTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGNNGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTT
   
Sequence Name 15-K107793-SALK_133064C-R210
GenBank Accession LN484687 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K107793-SALK_133064C-R210
GCGTCATTTGTTACCCAAAAGCTTCTTGTTTAGTAACTCCATATTGCTTCATATAACAAT
TTACTGCATTCGTGACATATCCTCTACTCATGTCATCCTAGCCAATAAATGTATAATAAA
TATGTACGTTAGATAGAACAAGAAAGTAGAAACATACCCCAGATGTGAAGAAATGATTTA
ATAGTTGTACCTCAAAACCAGTTATATCATTCTTAAGACGACATCTGGCACATAAAGTTT
GGATGAATTTAGGTCTGGACTTTAGCCATTCATAATCTTCTTTTGTAGCCTTCTGTTCCA
TACACATGAATATACCTGCCAAAGTCGAACACACTGAGATCTCCTCCTCACCAACTTCCA
TGTACTCCTCAAAGCTAGGCACATGGACAGCGTGTGCCCATTTGGCAAGATCAAAGTTGC
CTTTGGTAACTGTCTTAAACTGCATACTCAAAAGTATAGAACCATATTACGTGTACAAAC
TTTTTATTTTTCAAAATCGTTTTTCTATACCATAAGTTTCTTCTTTACAAAAAATATATA
CTTTTGATAAGCTCTTCTACAAAATATTAGTCCAACGAGTTCAATTGATTAATCATAATT
ACAGAGAAGAGTTTGATTGGTTACCTCTTCTATAGTGGCTTTCACAGTGTAAGATCTTCC
TTCTGGCTCAAGTTCTTTTTGAAACACTTCGAAAGTTTTCAATATAAAATTCAACACGAT
TTTTAAATAATCTGGTTGTTTATCCATGGCATCATTAGGAGCCCACCTATACATCATCAT
CATTTTCATCATGACCAACCACTA
Primers used for PCR 38xo - R212, show primer details
   
Sequence Name 83-K119306-SALK_133064C-38xo
GenBank Accession LN486264 [GenBank]
Sequencing primer gene specific primer, 38xo: 5'-GTCAAATATTCACGGTATTCTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K119306-SALK_133064C-38xo
CTAGTAGTAACCTACTGACCAAATTCGATCAAAGATTCAAGGTCAATCATATGTTCATCT
TGAATTAAGATTAAGACAAGTGGTAATAAAGTTTTGGTCATGAACTTACTATTACTCCTT
CAGGTCTATACATCACTCCACAAAAAAAGACAGTGTCCTTCTCTAAAGAATTAAGCATAG
ATATAAAAACAATTAATTTCACACGTCAAGTGAGGTAAGCACAAACATTGCAACTAAATT
GTTCCTTTGTTCTTGAAATATAATTTATTTGAAGCTTTGAGTGCAAATAATCTTAAAACA
AAAGAGCATATATAGAGTTTTGTCACTAACCTTAGATGATTATTGTAGTATGATAATGTA
TTAAAATGTTATAATTTAGTGTATCCATTTTCATTGGACACCCCTGAATTCACTTAATCA
AAAACATCATGATCAACCAACATTATAATAACATACACATCAAGTTTTTCATTACTTTTA
AAAAAGCATTATACAATCTAATTCTAGACAGTAGAAACCAAACACATGATCTGGTAAGAG
TAAACAAGAGAAAAATGTCATAGTGGTGAATTAAACACTCTACAACATATTTCAAAGTCT
TCAAAGACCGATACGATCGACAAACAGAGAAGTCATATACTCCTTGATCTTTCCTTGGGG
ATAGGTAAATCCTTCATATCCATTGTAGGTGATGAAGATCATACGTGCAAGATCAAAGGT
TGCTTTAAGAACGCAATGTCGCACACCCACCGTTGTCAGGAACTCTTCATTTAATATCTT
ATCAGCCTCTACGATGATTTTGTTAATAACACATTGCGGACAACAAATTAACGTTAATTT
AAACAGACAATCTGATATTGAGCGGA
   
Sequence Name 35-K119305-SALK_133064C-R214
GenBank Accession LN485229 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K119305-SALK_133064C-R214
GCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATATCAGATTGTCTGTTTAAATT
AACGTTAATTTGTTGTCCGCAATGTGTTATTAACAAAATCATCGTAGAGGCTGATAAGAT
ATTAAATGAAGAGTTCCTGACAACGGTGGGTGTGCGACATTGCGTTCTTAAAGCAACCTT
TGATCTTGCACGTATGATCTTCATCACCTACAATGGATATGAAGGATTTACCTATCCCCA
AGGAAAGATCAAGGAGTATATGACTTCTCTGTTTGTCGATCGTATCGGTCTTTGAAGACT
TTGAAATATGTTGTAGAGTGTTTAATTCACCACTATGACATTTTTCTCTTGTTTACTCTT
ACCAGATCATGTGTTTGGTTTCTACTGTCTAGAATTAGATTGTATAATGCTTTTTTAAAA
GTAATGAAAAACTTGATGTGTATGTTATTATAATGTTGGTTGATCATGATGTTTTTGATT
AAGTGAATTCAGGGGTGTCCAATGAAAATGGATACACTAAATTATAACATTTTAATACAT
TATCATACTACAATAATCATCTAAGGTTAGTGACAAAACTCTATATATGCTCTTTTGTTT
TAAGATTATTTGCACTCAAAGCTTCAAATAAATTATATTTCAAGAACAAAGGAACAATTT
AGTTGCAATGTTTGTGCTTACCTCACTTGACGTGTGAAATTAATTGTTTTTATATCTATG
CTTAATTCTTTAGAGAAGGACACTGTCTTTTTTTGTGGAGTGATGTATAGACCTGAAGGA
GTAATAGTAAGTTCATGACCAAAACTTTATTACCACTTGTCTTAATCTAATTCCAGATGA
ACATATGATTGACCTTGAATCTTTGATCGAATTTGGTCAGTAGTGTTACTACTAGGGTAA
TGATG

Last Updated on 06.12.2018 16:11