Sequences from confirmation amplicon


Confirmation sequences for line SALK_133630 and hit At2g34410 are shown.
Line ID SALK_133630
Locus At2g34410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14521552
Primers used for PCR CG88 - R212, show primer details
   
Sequence Name 54-K107035-SALK_133630C-R214
GenBank Accession LN485595 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107035-SALK_133630C-R214
GCGGGGGTCATACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGCC
TTCAGTTTAAAACCACCCCAGTATATTTCTCAGTTTCACATCTGGTTAAGGTCAGCGAGA
AACTGTTTCCTTTCTCCCAATTATGTAGGCAAGAGACCATTTGTCTGAAGGATCTAGGAA
TGAAATTTTAACTGCAAATTATGTGTTCTGCAGATCGAATGTGCCAAATGGACAGCCTAA
GTGGCTATTATGCATTATTCCAGAATACCCAATGCTCAACTTCATGCTCGTCACGGCCAT
CTATGTCTTGGTAAAGCAATTGTAGAATCCATGAAGAAATCCTGTATTCACGATACGTTC
TATTATTGTAATATGTTCTTTTGATTTATTTTCTTCAGGTGTCCCACCGACTTTTCGAGC
TTACAAACACGTTAAAGTCTGTTTTCATACCAACAAAAGACGACAAGAGGCTGCTCCACA
ATGTTCTCGCTGGAGCTGCCATCTCGTTCTGTTTATATTTAACATCTCTCATTCTTCTCC
AGATCCCAC
   
Sequence Name 62-K107036-SALK_133630C-CG88
GenBank Accession LN485783 [GenBank]
Sequencing primer gene specific primer, CG88: 5'-CCTTACATGGTTAGTGTGGGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K107036-SALK_133630C-CG88
TATAAACGAACGAGATGGCAGCTCCGCGAGAACATTGTGGAGCAGCCTCTTGTCGTCTTT
TGTTGGTATGAAAACAGACTTTAACGTGTTTGTAAGCTCGAAAAGTCGGTGGGACACCTG
AAGAAAATAAATCAAAAGAACATATTACAATAATAGAACGTATCGTGAATACAGGATTTC
TTCATGGATTCTACAATTGCTTTACCAAGACATAGATGGCCGTGACGAGCATGAAGTTGA
GCATTGGGTATTCTGGAATAATGCATAATAGCCACTTAGGCTGTCCATTTGGCACATTCG
ATCTGCAGAACACATAATTTGCAGTTAAAATTTCATTCCTAGATCCTTCAGACAAATGGT
CTCTTGCCTACATAATTGGGAGAAAGGAAACAGTTTCTCGCTGACCTTAACCAGATGTGA
AACTGAGAAATATACTGGGGTGGTTTTAAACTGAAGGCGGGAAACGACAATCTGATCATG
AGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTT
ACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTGGAG
TTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTC
Primers used for PCR CG87 - R204, show primer details
   
Sequence Name 55-K107035-SALK_133630C-R210
GenBank Accession LN485615 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107035-SALK_133630C-R210
CGTCATTTGTTTCACCACATTGCGGACGCCCCAATTTGTCTAAGCTACGAAGATATGACA
GTCATGTCATATTAGAGTTGTGTACAGAATAAAACACTAAGCTTGGTTAGAATGAGACTA
AGATTGAGAAACATACGCAAATAGTGTCATGGAGAAATTACGCAGCTGTTGTGTAGAATT
TCGCAGACAGATGTAGACGCTAAGACAAAATAATCAAAGCTAAAGTCAGAATTTAGAGAG
TAAGACATCTCAAAGTTTAATGTTTGGGGTGAAAGAAAACGGGATTTGATACTAACGTTA
TTGGAATCCACGATGTGTAGGGATGATATTTGTTGTATGTAACCTTGTCAAGCTTGTATA
TGTATTCATACCATAGGTAACCAACCTGAACAATATAGGGAACAACTTGGGCATCAGAAT
AATATCAAATACATAAGATCCATAAGCCAGAGAGAACCCAAACTCACAAATGAGGAAATT
GCAATTATGCTTGTCTTTATTGACATCTTTCTCTTGGCATCACACTCCTCCAATTTCTCC
ATCCATCTCTCTACCTGAAATGAAAATGTCAAAAAATATTTTAATCGTAGGGTTCATGAG
AAGATATGAAAAAACGAGAAGGCTGTAGGTTCAAGAGTTACAGTGGGATGGAAATAGGCA
TATATCATTCCAATGATCCATATGTAGCGGTCAAGTCCTGATCTGAAGTGCCATTCATGT
AAAAG
   
Sequence Name 63-K107036-SALK_133630C-CG87
GenBank Accession LN485811 [GenBank]
Sequencing primer gene specific primer, CG87: 5'-CTTTTACATGAATGGCACTTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107036-SALK_133630C-CG87
TGGATGATATATGCCTATTTCCTCCCACTGTAACTCTTGAACCTACAGCCTTCTCGTTTT
TTCATATCTTCTCATGAACCCTACGATTAAAATATTTTTTGACATTTTCATTTCAGGTAG
AGAGATGGATGGAGAAATTGGAGGAGTGTGATGCCAAGAGAAAGATGTCAATAAAGACAA
GCATAATTGCAATTTCCTCATTTGTGAGTTTGGGTTCTCTCTGGCTTATGGATCTTATGT
ATTTGATATTATTCTGATGCCCAAGTTGTTCCCTATATTGTTCAGGTTGGTTACCTATGG
TATGAATACATATACAAGCTTGACAAGGTTACATACAACAAATATCATCCCTACACATCG
TGGATTCCAATAACGTTAGTATCAAATCCCGTTTTCTTTCACCCCAAACATTAAACTTTG
AGATGTCTTACTCTCTAAATTCTGACTTTAGCTTTGATTATTTTGTCTTAGCGTCTACAT
CTGTCTGCGAAATTCTACACAACAGCTGCGTAATTTCTCCATGACACTATTTGCGTATGT
TTCTCAATCTTAGTCTCATTCTAACCAAGCTTAGTGTTTTATTCTGTACACAACTCTAAT
ATGACATGACTGTCATATCTTCGTAGCTTAGACAAATTGGGGCGTCCGCAATGTGGTGTA
AACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGT
GGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTG
AGAGAGTTGCAGC

Last Updated on 06.12.2018 16:11