Sequences from confirmation amplicon


Confirmation sequences for line SALK_136073c and hit At1g49490 are shown.
Line ID SALK_136073c
Locus At1g49490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18319189
Primers used for PCR 38mv - LBb3, show primer details
   
Sequence Name 31-K114624-SALK_136073C-38mv
GenBank Accession LN485150 [GenBank]
Sequencing primer gene specific primer, 38mv: 5'-TAGATGATGATAGTCTCACGGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114624-SALK_136073C-38mv
CCGGACATTTACACCAGAGCTTGGCCTTATGACGGACCTAGCTTTATTTCACATCAATTC
GAATCGGTTTTGTGGGATCATCCCCAAAAGTTTATCGAAACTCGCTTTGATGTACGAATT
CGATGTTAGCAACAATCGATTCGTTGGTCAGTTCCCTGAAGTTTCACTCTCATGGCCTTC
TTTGAAGTTTCTTGATCTAAGATACAACGAATTTGAAGGAAGCTTGCCATCAGAAATCTT
TGATAAAGATCTTGATGCAATTTTCTTGAACAACAATCGGTTTGAATCGGTTATCCCCGG
TACAATTGGGAAATCGAAAGCATCGGTTGTGACATTCGCAAACAATAAATTCAGTGGTTG
TATCCCAAAATCGATTGGTAATATGAAGAACCTCAATGAGATTGTCTTCACTGGAAACAA
TTTAACCGGTTGTTTCCCAAACGAGATTGGTTTGCTTAACAATGTGACGGTGTTTGATGC
AAGCAAGAACGGTTTTGTCGGTAGTTTACCGTCAACCTTGTCCGGTTTAGCTAGCGTTGA
GCAGTTAGATTTATCGCATTGACAAACAAATTGACGCTNAGACAACTTAATAACACATTG
CGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCC
CAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 15-K114623-SALK_136073C-R210
GenBank Accession LN484701 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114623-SALK_136073C-R210
GTTTGTCATGCGATAATCTAACTGCTCAACGCTAGCTAAACCGGACAAGGTTGACGGTAA
ACTACCGACAAAACCGTTCTTGCTTGCATCAAACACCGTCACATTGTTAAGCAAACCAAT
CTCGTTTGGGAAACAACCGGTTAAATTGTTTCCAGTGAAGACAATCTCATTGAGGTTCTT
CATATTACCAATCGATTTTGGGATACAACCACTGAATTTATTGTTTGCGAATGTCACAAC
CGATGCTTTCGATTTCCCAATTGTACCGGGGATAACCGATTCAAACCGATTGTTGTTCAA
GAAAATTGCATCAAGATCTTTATCAAAGATTTCTGATGGCAAGCTTCCTTCAAATTCGTT
GTATCTTAGATCAAGAAACTTCAAAGAAGGCCATGAGAGTGAAACTTCAGGGAACTGACC
AACGAATCGATTGTTGCTAACATCGAATTCGTACATCAAAGCGAGTTTCGATAAACTTTT
GGGGATGATCCCACAAAACCGATTCGAATTGATGTGAAATAAAGCTAGGTCCGTCATAAG
GCCAAGCTCTGGTGGTAAATGTCCGGCTATATCTGCATGGTTAAGGTCAACTCCAGCTAC
GACCGTGAGACACAANCCATCT

Last Updated on 06.12.2018 16:11