Sequences from confirmation amplicon


Confirmation sequences for line SALK_136802c and hit At3g23390 are shown.
Line ID SALK_136802c
Locus At3g23390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8375434
Primers used for PCR 57fr - LBb3, show primer details
   
Sequence Name 29-K112129-SALK_136802C-57fr
GenBank Accession LN485106 [GenBank]
Sequencing primer gene specific primer, 57fr: 5'-CATATCTATTGGGCTTCGATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112129-SALK_136802C-57fr
TTCTTGGTTCAACGAAACAAAACTTAAAAGTAAATCATTTTTTTCTACTGAATTTTGTTT
TTGATGCTTTTGATTTCATTTGATCACTTCAACTTTAGTTCCAGGGTCTTGACGATTTAA
TTCAAAAAGCAAAAAAATCAATAGGAAACAAAAACTCATAAAGGACTTTGACATACAGAT
GGGCCCATTGTTTATGACCAATCCTTATACTATATATGGGCCTTATTAGTTAAACCTAAG
GCCCAAAGTCAGATTAGGGTTTTCAGAAAGTGTACTATAAATTCTTCTTCTTTAAACAAC
TTCGTCTAGTGGAACGACGACGGCACAAAAGCTTCACCGGAGATCAGAGACGCGAAAATG
GTAAATTGTTTCTTCTCTTTCGGACAACTTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 13-K112128-SALK_136802C-R210
GenBank Accession LN484639 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K112128-SALK_136802C-R210
TTTACCATTTTCGCGTCTCTGATCTCCGGTGAAGCTTTTGTGCCGTCGTCGTTCCACTAG
ACGAAGTTGTTTAAAGAAGAAGAATTTATAGTACACTTTCTGAAAACCCTAATCTGACTT
TGGGCCTTAGGTTTAACTAATAAGGCCCATATATAGTATAAGGATTGGTCATAAACAATG
GGCCCATCTGTATGTCAAAGTCCTTTATGAGTTTTTGTTTCCTATTGATTTTTTTGCTTT
TTGAATTAAATCGTCAAGACCCTGGAACTAAAGTTGAAGTGATCAAATGAAATCAAAAGC
ATCAAAAACAAAATTCAGTAGAAAAAAATGATTTACTTTTAAGTTTTGGTTTCGTTTGAA
CCAAGAAATAAAAACCTATTATAACATGTAAACGCAACAAACAATCGAAGCCANTTAGAT
AT

Last Updated on 06.12.2018 16:11