Sequences from confirmation amplicon


Confirmation sequences for line SALK_137069 and hit At2g32510 are shown.
Line ID SALK_137069
Locus At2g32510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13798902
Primers used for PCR 41cv - LBb3, show primer details
   
Sequence Name 25-K109252-SALK_137069C-41cv
GenBank Accession LN484997 [GenBank]
Sequencing primer gene specific primer, 41cv: 5'-TGATAGAGATGGTTACGGGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K109252-SALK_137069C-41cv
GGTTTCGGTTCTTTATCGGGTCGGGTATTCGAGTGAAACTCCGGAGCTTCCTTGTTTGCT
TGCGGAAGAAGCAAAAGATTTTTTGGAAAAGTGTTTGAAGAGGGAAGCAAATGAAAGATG
GACAGCGACACAACTCCTAAACCATCCTTTTTTGACAACTAAACCAGACATTGAACCAGT
TCTGGTTCCCGGTTTGATTTCAAACTCACCGACAAGTGTGACAGATCAAACGTTTTGGAG
ATCAGTAGAGGAAGAGGAAGAAGAAGAAACAGAGGAAATACAGAAGGATTCGAGAGATCT
TGATCGTTTAAGCTTGTGGGGTTGTTACTCGGAGAGGATCGGACGGCTGAAGTGTGTTGG
TGGTTTGGATGGGACCAGATGTGATATGGAGGGTGGGGATTGGATTATGGTGAGACNNCT
TGATCTNNCNCNTTGACGCTTATACAACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAACAAGCGGTCCACGCTGGTTTGCTCCAGCAGGGCGAAATCCTG
TTTGATGAAAAATCCGAAATCGGCAAAA
   
Sequence Name 35-K109255-SALK_137069C-R210
GenBank Accession LN485216 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K109255-SALK_137069C-R210
TTAGATCAGTTGTCTCACCATAATCCAATCCCCACCCTCCATATCACATCTGGTCCCATC
CAAACCACCAACACACTTCAGCCGTCCGATCCTCTCCGAGTAACAACCCCACAAGCTTAA
ACGATCAAGATCTCTCGAATCCTTCTGTATTTCCTCTGTTTCTTCTTCTTCCTCTTCCTC
TACTGATCTCCAAAACGTTTGATCTGTCACACTTGTCGGTGAGTTTGAAATCAAACCGGG
AACCAGAACTGGTTCAATGTCTGGTTTAGTTGTCAAAAAAGGATGGTTTAGGAGTTGTGT
CGCTGTCCATCTTTCATTTGCTTCCCTCTTCAAACACTTTTCCAAAAAATCTTTTGCTTC
TTCCGCAAGCAAACAAGGAAGCTCCGGAGTTTCACTCGAATACCCGACCCGATAAAGAAC
CGAAACCGGATCCTCCCTCGAATCTGCCTTCGTCCACGGTGGAGAACCCGTAACCATCTC
TATC

Last Updated on 06.12.2018 16:11