Sequences from confirmation amplicon


Confirmation sequences for line SALK_138548c and hit At3g07510 are shown.
Line ID SALK_138548c
Locus At3g07510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2393992
Primers used for PCR BL15 - R204, show primer details
   
Sequence Name 33-K104740-SALK_138548C-BL15
GenBank Accession LN485180 [GenBank]
Sequencing primer gene specific primer, BL15: 5'-TGAGTAAGCAAACCCTCTCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K104740-SALK_138548C-BL15
CATTGCGTTTCTTTTTTTCCTTTCCGAGCAGATCTGAGCCGAAGGTCATTAAATGACCGG
CGCTGAGAACGGCGTGAGAATGACGTCACTAGAGGCGTCTTCAACATCCTCCCTCGCCAC
CACAAGTGTCCACGTAAGCGCTCTCGACGGACTCGTGAACGTGAACTCTCTCTTCACCAT
CGCCGTCTTCGTTGGTCTTTCCTTAGCGACTCCAGGACAACACAGCCTCGAACAACGATC
CAGCTGCGACGCAAGTGCTGACGTCGCCAAGAAGCTACTGGTCTTCGAAGTCGTCTCCTT
CAGCTTCTTCCTCTTCTCCTCTCTTGTAGCTCAGGGTCTTAAGCTTGCCTTAAACCTTCT
TAATAGCAAAGACGTCAACGAGATTTTCAGAGCTCACATCAATATCAAGGTTTTGAGATG
GGGAATGATGGCTTCTGCTGTCGGATCTGTAATGGGCTGTCTCTTCTTGATGCTCTCTAT
GGTTAATGTGTTGTCTTAGGTTTACCCGTCAATATATTGTGGTGTAAACAAATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCAC
   
Sequence Name 92-K104792-SALK_138548C-R210
GenBank Accession LN486344 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K104792-SALK_138548C-R210
GTCATTTGTTACCCACAATATATTGACGGGTAAACCTAAGACAACACATTCCCCTTAGAG
AGCATCAAGAAGAGACAGCCCATTACAGATCCGACAGCAGAAGCCATCATTCCCCATCTC
AAAACCTTGATATTGATGTGAGCTCTGAAAATCTCGTTGACGTCTTTGCTATTAAGAAGG
TTTAAGGCAAGCTTAAGACCCTGAGCTACAAGAGAGGAGAAGAGGAAGAAGCTGAAGGAG
ACGACTTCGAAGACCAGTAGCTTCTTGGCGACGTCAGCACTTGCGTCGCAGCTGGATCGT
TGTTCGAGGCTGTGTTGTCCTGGAGTCGCTAAGGAAAGACCAACGAAGACGGCGATGGTG
AAGAGAGAGTTCACGTTCACGAGTCCGTCGAGAGCGCTTACGTGGACACTTGTGGTGGCG
AGGGAGGATGTTGAAGACGCCTCTGGTGACGTCATTCTCACGCCGTTCTCAGCGCCGGTC
ATGTAGTTAACCTTCGGCTCAGATCTGCTCGGAAAGGGAAAAAAAGAAACGCAATGGTTA
AAAAAAAGATAGATTGATTTTAGGGAAGAGAGAGGGTTTGCTTACTC
Primers used for PCR 94yp - LBb3, show primer details
   
Sequence Name 47-K122661-SALK_138548C-94yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 94yp: 5'-AGGGTTTGGTTAGGTATTCGGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K122661-SALK_138548C-94yp
CTTTGCTCATTTGTTTTGAGTTGAGATGTTTGTGTGTTGTGTTGTGGCAGTGGAAGGGAA
CTCTTTACCCGAAGAAGATGTAGAGATGATGGAGAATTCAGATGTAATGAAGACAGTCAC
AGATGAAGCTTCTCCAATGGTGGAGCCGTTCATTGGTATGGAGTTTGAATCAGAAGAGGC
TGCAAAGTCTTTCTATGACAATTACGCTACATGCATGGGTTTCGTTATGCGAGTGGATGC
TTTCAGGAGGTCAATGCGAGATGGGACTGTGGTGTGGCGCAGACTCGTGTGTAATAAAGA
AGGATTTCGTAGGTCGAGACCTCGGAGAAGCGAGAGTAGAAAGCCTCGGGCTATAACAAG
AGAAGGGTGTAAAGCTCTGATTGTTGTGAAAAGAGAGAAATCCGGAACATGGCTTGTTAC
TAAATTTGAGAAAGAGCATAATCATCCTCTCTTGCCTCTCTCCCCTAATGTTCGCCGGAA
TTTTCAGCTCCCTCAAACACCGGTACGTCTACGCACTTTTCCTTGGGGTAAAAGTAAAAC
AGTGATTATTGGCATTGATCTAAGAGTTACCAAGAACATAAAGCTCAAAAAATGATATCG
AAATCTTGTACAAATGAACTTTTCAGGACGAGAAAGACGCAAAGATTAGGGAGCTGAGTG
CAGAGCTGAGCCGAGAGAGAAGGCGTTGTACTGCTTTGCAACAGCAGCTTGATATGGTCC
TTAAGGAGATGGAAGAACATTCCAACCATCTCACCATTAACATCAATAGTGTTATCCAAA
GTGTTAGAGACATTGAGTCCAATACTTTCACCAAACAACAAACATAATGCTTCATTTCTG
TTCATAAAAGCTAACATCAAATTCTCATA
   
Sequence Name 45-K122660-SALK_138548C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K122660-SALK_138548C-R210
CATTTGTTTACGCAAATCGGCGGCTCAAGCTGTTGCAACGCTCGTCACTCTGGTTTCCTC
TGCTCTGCTGATTTACATCTCTACCGCCATTTACGCTTTCTGGCATTGATGCTATGTCAA
AATTGGATAATGTCCATGGCTTTGTTTTGATTAGCCAATGTGTAATTTCATCATCAATGC
CTTCCAATCTAGTTTTTTGTTTTTTTGTTTCGGAGAATGAAGAATCAATCACAAGATACA
GAACATTGGATTATGAGAATTTGATGTTAAGCTTTTATGAACAGAAATGAAGCATTATGT
TTGTTGTTTGGTGAAAGTATTGGACTCAATGTCTCTAACACTTTGGATAACACTATTGAT
GTTAATGGTGAGATGGTTGGAATGTTCTTCCATCTCCTTAAGGACCATATCAAGCTGCTG
TTGCAAAGCAGTACAACGCCTTCTCTCTCGGCTCAGCTCTGCACTCAGCTCCCTAATCTT
TGCGTCTTTCTCGTCCTGAAAAGTTCATTTGTACAAGATTTCGATATCATTTTTTGAGCT
TTATGTTCTTGGTAACTCTTAGATCAATGCCAATAATCACTGTTTTACTTTTACCCCAAG
GAAAAGTGCGTAGACGTACCGGTGTTTGAGGGAGCTGAAAATTCCGGCGAACATTAGGGG
AGAGAGGCAAGAGAGGATGATTATGCTCTTTCTCAAATTTAGTAACAAGCCATGTTCCGG
ATTTCTCTCTTTTCACAACAATCAGAGCTTTACACCCTTCTCTTGTTATAGCCCGAGGCT
TTCTACTCTCGCTTCTCCGAGGTCTCGACCTACGAAATCCTTCTTTATTACACACGAGTC
TGCGCCACACAACAGTCCCATCTCGCATTGACCTCCTGAAACATCCACTCGCATAACAAA

Last Updated on 06.12.2018 16:11