Sequences from confirmation amplicon


Confirmation sequences for line SALK_141756c and hit At1g28440 are shown.
Line ID SALK_141756c
Locus At1g28440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9999751
Primers used for PCR 28fx - LBb3, show primer details
   
Sequence Name 68-K111812-SALK_141756C-28fx
GenBank Accession LN485953 [GenBank]
Sequencing primer gene specific primer, 28fx: 5'-GGCTATGTATGGCTTCTTAGATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K111812-SALK_141756C-28fx
GGTTCTCTTCAGTACAGGACTTTCAGAAAGCAAGAGCCATGGAGAGATCTAAGTGGACTC
TAATGTCGTTCCACAAACTCGGGTTCAGTGAGCATGAGATTCTTGAAAGCTTGGATGAAG
ATAATGTGATTGGAGCTGGAGCTTCAGGTAAAGTTTACAAGGTTGTACTCACCAATGGGG
AAACTGTTGCGGTTAAGCGTTTATGGACAGGTTCTGTTAAGGAAACTGGAGATTGTGATC
CAGAGAAAGGTTACAAACCTGGAGTTCAAGATGAGGCTTTTGAAGCTGAAGTTGAGACAT
TGGGTAAGATTAGGCATAAGAACATTGTGAAGCTATGGTGTTGCTGTTCTACAAGAGACT
GCAAGCTCTTGGTTTATGAGTACATGCCTAATGGTAGTTTGGGAGATTTGCTTCATAGCA
GTAAAGGAGGAATGTTGGGATGGCAAACGAGGTTTAAGATTATCTTAGATGCGGCTGAGG
GGCTTTCGTATCTTCACCATGATTCTGTTCCTCCGATTGTGCATAGAGATATTAAGTCAA
ACAATATTTTGATCGATGGAGATTATGGTGCAAGAGTTGCTGATTTTGGTGTGGCTAAAG
CTGTCGACTTGACCGGAAAAGCTCCTAAATCGATGTCAGTGATCGCTGGTTCATGCGGTT
ATATCGCACCAGGTTAGTCTTATGATTCAAGTTAAATGTGCTTTGTTTAATCAACAGTTT
TGCTTAAGGTAATAATGCTCATTTGGTACATGTTGCACGGAGATTCAGATGAACCACATT
TAAGTTTTATTAGTTGAGACTTAAATGAGCATTCACTATCTATTGAGACATGTCTGAAAC
CATGGCTCCACAACTTCTCACTTGTCCATTAATGGAATTTGAAACTTTTTGTTTGTTTGT
CTGTTTCCCTCCATAACTTTCATGGTGATCCATTAATGTCACATTGACGCTNAGACAACT
TAAATACACATTGCGGACGTTTTTAATGTACCTGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTT
   
Sequence Name 58-K111811-SALK_141756C-R210
GenBank Accession LN485688 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K111811-SALK_141756C-R210
TGGATCACCATGAAAGTTATGGAGGGAAACAGACAAACAAACAAAAAGTTTCAAATTCCA
TTAATGGACAAGTGAGAAGTTGTGGAGCCATGGTTTCAGACATGTCTCAATAGATAGTGA
ATGCTCATTTAAGTCTCAACTAATAAAACTTAAATGTGGTTCATCTGAATCTCCGTGCAA
CATGTACCAAATGAGCATTATTACCTTAAGCAAAACTGTTGATTAAACAAAGCACATTTA
ACTTGAATCATAAGACTAACCTGGTGCGATATAACCGCATGAACCAGCGATCACTGACAT
CGATTTAGGAGCTTTTCCGGTCAAGTCGACAGCTTTAGCCACACCAAAATCAGCAACTCT
TGCACCATAATCTCCATCGATCAAAATATTGTTTGACTTAATATCTCTATGCACAATCGG
AGGAACAGAATCATGGTGAAGATACGAAAGCCCCTCAGCCGCATCTAAGATAATCTTAAA
CCTCGTTTGCCATCCCAACATTCCTCCTTTACTGCTATGAAGCAAATCTCCCAAACTACC
ATTAGGCATGTACTCATAAACCAAGAGCTTGCAGTCTCTTGTAGAACAGCAACACCATAG
CTTCACAATGTTCTTATGCCTAATCTTACCCAATGTCTCAACTTCAGCTTCAAAAGCCTC
ATCTTGAACTCCAGGTTTGTAACCTTTCTCTGGATCACAATCTCCAGTTTCCTTAACAGA
ACCTGTCCATAAACGCTTAACCGCAACAGTTTCCCCATTGGTGAGTACAACCTTGTAAAC
TTTACCTGAAGCTCCAGCTCCNATCACATTATCTTCATCCAAGCTNTCAAGAATCTCATG
CTCACTGAACCCGAGTTTGTGGAACGACATTAGAGTCCACTTAGATCTCTCCATGGCTCT
NGCTTTCTTGAAAGTCCTGTACTTGAAGTAGAACCAAGCAACACCCGCAAGAAGCACCAT
CGCAGCANGTACGA

Last Updated on 06.12.2018 16:11