Sequences from confirmation amplicon


Confirmation sequences for line SALK_143700c and hit At2g37050 are shown.
Line ID SALK_143700c
Locus At2g37050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15570599
Primers used for PCR 07as - R204, show primer details
   
Sequence Name 07-K107313-SALK_143700C-07as
GenBank Accession LN484448 [GenBank]
Sequencing primer gene specific primer, 07as: 5'-TCCCTGCTGATTCAAGAAAGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K107313-SALK_143700C-07as
AGATAGATATCTCATTAGAGCTACTTTCTTGTATGGTAATTTCGACAACAGCAACAATGT
GTATCCCAAATTCGATATCTCCCTCGGGGCGACTCATTGGGCGACCATTGTTATCTCTGA
AACCTATATAATCGAAACTGCAGAACTGGTGTTTCTGGCTTCTTCTCCTACTGTTAGTGT
TTGTTTGTCAAATGCCACAACTGGACAGCCGTTTATATCTACTCTTGAGCTTAGGCAGTT
GAGTGGTTCGATGTATGGTTCGATGCTATCTGAAGACCGGTTTTACCTGAGTGTTGCAGC
ACGGATAAATTTCGGGGCTGAAAGTGAAGCTTCTGTGAGGTCAGTATACAGTTCATAATC
TGTTTGTTGTTTCAGCATTGTGATTTATCAGGATGAGTTAAGGAAATGAGTTGGTATTGC
AGGTACCCGGATGATCCGTATGATAGAATATGGGAATCTGATTTGCAAAAGAAGCCTAAT
TATCTTGTGGATGTTGCTGCTGGGACTGTGCGAGTGTCGACTACATTGCCAATCGAAAGC
AGGGTCGATGACAGACCGCCTCAGAAGGTTATGCAGACGGCTGTGGTTGGAACAAACGGG
TCCTTAACTTACAGAATGAACCTAGATGGCTTCCCTGGTTTTGGTTGGGCATTCACATAC
TTTGCTGAAATCGAGGATTTGGCTGAGGATGAGTCAAGGAAGTTTAGATTAGTTCTACCT
GAGCAGCCTGAGTATAGCAAATCGGTTGTTAACATCAAGGAAAATACACAGAGACCGTAT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTGCA
   
Sequence Name 47-K107289-SALK_143700C-R210
GenBank Accession LN485430 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107289-SALK_143700C-R210
GCGTCATTTGTTTATACGGTCTCTGTGTATTTTCCTTGATGTTAACAACCGATTTGCTAT
ACTCAGGCTGCTCAGGTAGAACTAATCTAAACTTCCTTGACTCATCCTCAGCCAAATCCT
CGATTTCAGCAAAGTATGTGAATGCCCAACCAAAACCAGGGAAGCCATCTAGGTTCATTC
TGTAAGTTAAGGACCCGTTTGTTCCAACCACAGCCGTCTGCATAACCTTCTGAGGCGGTC
TGTCATCGACCCTGCTTTCGATTGGCAATGTAGTCGACACTCGCACAGTCCCAGCAGCAA
CATCCACAAGATAATTAGGCTTCTTTTGCAAATCAGATTCCCATATTCTATCATACGGAT
CATCCGGGTACCTGCAATACCAACTCATTTCCTTAACTCATCCTGATAAATCACAATGCT
GAAACAACAAACAGATTATGAACTGTATACTGACCTCACAGAAGCTTCACTTTCAGCCCC
GAAATTTATCCGTGCTGCAACACTCAGGTAAAACCGGTCTTCAGATAGCATCGAACCATA
CATCGAACCACTCAACTGCCTAAGCTCAAGAGTAGATATAAACGGCTGTCCAGTTGTGGC
ATTTGACAAACAAACACTAACAGTAGGAGAAGAAGCCAGAAACACCAGTTCTGCAGTTTC
GATTATATAGGTTTCAGAGATAACAATGGTCGCCCAATGAGTCGCCCCGAGGGAGATATC
GAATTTGGGATACACATTGTTGCTGTTGTCGAAATTACCATACAAGAAAGTAGCTCTAAT
GAGATATCTATTCCTACTCGTGACATTGAGAGTGTAGCAATACTTTCTTG

Last Updated on 06.12.2018 16:11