Sequences from confirmation amplicon


Confirmation sequences for line SALK_144605c and hit At4g29000 are shown.
Line ID SALK_144605c
Locus At4g29000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14295096
Primers used for PCR 24kh - LBb3, show primer details
   
Sequence Name 78-K113949-SALK_144605C-24kh
GenBank Accession LN486191 [GenBank]
Sequencing primer gene specific primer, 24kh: 5'-TATCTGCTAAAAGGGACCTTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113949-SALK_144605C-24kh
CTCTTGGACAACTAATAACTTGTGAACCCAGAAATTTCACCTGTATACAAACTTTGATGG
AACTGACGATGCATTGCCACCAGCACGAGAAACAGGTGATGGAGATGTGCCTGAGGTTGG
TCCACCAGTTCTTCCATTATTTACCTAAATACAAATAATAAGATTTCACTTCACTAAGCC
AACAGCAAGAGAAAAATATATTTTTCAGTTCTATAAATGGTTACCAAAAAGCATATATAT
GCTAAAATGGTAAATTGGAACCTGTGGAAAGTGGCTAAGCCTAGATGAATCTTTAATTGC
TTGGTTGAACAAAATCTCTTGACCTTTCCTTCTCTTAGGTGCTGGAGAAGGAGCAAAGCC
GGAGGAACCAACAGCTCCAGTAATGGCTGCATTTGCTGCTTGTTGAAGATAAGCCATGTG
GTTGGAATGTTCACCGTGAAACAAAGCTTGTCTCTCTTCACTTCCTTCAAAGTTCTTGCA
ATCCAAGCATTTGCAGTTCTCGGAACAAAGAATATTTGCTTGAAAGCACTCGCAATACTT
CTTAAGGCATCCTGATTTTTTGCAGTGGCATCCTTTGACGCTTAGACAACTTAATAACAC
ATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTT
   
Sequence Name 67-K113948-SALK_144605C-R210
GenBank Accession LN485934 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113948-SALK_144605C-R210
CAAAATCAGGATGCCTTAAGAAGTATTGCGAGTGCTTTCAAGCAAATATTCTTTGTTCCG
AGAACTGCAAATGCTTGGATTGCAAGAACTTTGAAGGAAGTGAAGAGAGACAAGCTTTGT
TTCACGGTGAACATTCCAACCACATGGCTTATCTTCAACAAGCAGCAAATGCAGCCATTA
CTGGAGCTGTTGGTTCCTCCGGCTTTGCTCCTTCTCCAGCACCTAAGAGAAGGAAAGGTC
AAGAGATTTTGTTCAACCAAGCAATTAAAGATTCATCTAGGCTTAGCCACTTTCCACAGG
TTCCAATTTACCATTTTAGCATATATATGCTTTTTGGTAACCATTTATAGAACTGAAAAA
TATATTTTTCTCTTGCTGTTGGCTTAGTGAAGTGAAATCTTATTATTTGTATTTAGGTAA
ATAATGGAAGAACTGGTGGACCAACCTCAGGCACATCTCCATCACCTGTTTCTCGTGCTG
GTGGCAATGCATCGTCAGTTCCATCAAAGTTTGTATACAGGTGAAATTTCTGGTGTTCAC
AAGTTATTTAGTTTGATCCAAGAGCTGTCTAAAACTGACTTGTACGTTGTAATTTTTTGA
AGGTCCC

Last Updated on 06.12.2018 16:11