Sequences from confirmation amplicon


Confirmation sequences for line SALK_145146 and hit At2g46260 are shown.
Line ID SALK_145146
Locus At2g46260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18996537
Primers used for PCR 09bt - LBb3, show primer details
   
Sequence Name 23-K108287-SALK_145146C-09bt
GenBank Accession LN484938 [GenBank]
Sequencing primer gene specific primer, 09bt: 5'-TTGTCGTACCTTGTGCACTAATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K108287-SALK_145146C-09bt
TTCCTCATTCATTAGAAACAGCTACACAGGGTAAGGAAAACAAGAAGCAAAAGATAGCAT
TACATGCGAGATGAACAAGAGCAAACAATAAGAACATAGAAGAAGAAAAAAGGTTAATAC
CTTGTAGAAAAAGGGGCTTTTTGCGGCCAAGATAGGAGAACTAATATGCAGTTCTTTAAC
TCTAACAACAGTAGAACAATCAATACCCCAGTTTGGCTCACTAGATGCATCATCATCACC
TACAAAAACCATTAATAAACAAAGCCATCACGTTTCAGACAAATGACTTCAACAAAAAAC
GCTCTTCTGAAAATTGTTAAGTATCACAAATTCAAACCCAGTCACTCGAGAGACAATGTG
AAATTTAGAACGGTACCTGATAAAGCCTCTTCAACCATTGCCTCTCCTTCATCGTCAAGA
TTATCACCACCAGGAGCATCATCCATATCAGGTTGGTTGTTATCGGTTAAAATCTGTTCT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATGGGCAAAA
   
Sequence Name 07-K108286-SALK_145146C-R210
GenBank Accession LN484449 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K108286-SALK_145146C-R210
CCGATAACACCACCTGATATGGATGATGCTCCTGGTGGTGATAATCTTGACGACGTTTGA
GAGGCAATGGTTGAAGAGGCTTTATCAGGTACCGTTCTATATTTCACATTGTCTCTCGAG
TGACTGGGTTTGAATTTGTGATACTTAACAATTTTCAGAAGAGCGTTTTTTGTTGAAGTC
ATTTGTCTGAAACGTGATGGCTTTGTTTATTAATGGTTTTTGTAGGTGATGATGATGCAT
CTAGTGAGCCAAACTGGGGTATTGATTGTTCTACTGTTGTTAGAGTTAAAGAACTGCATA
TTAGTTCTCCTATCTTGGCCGCAAAAAGCCCCTTTTTCTACAAGGTATTAACCTTTTTTC
TTCTTCTATGTTCTTATTGTTTGCTCTTGTTCATCTCGCATGTAATGCTATCTTTTGCTT
CTTGTTTTCCTTACCCTGTTGTAGCTGTTTTCTAATGGAATGAGGGAATCAGAACAAAGA
CATGTAACCCTTAGAATTAGTGCACAAGGTACGACA

Last Updated on 06.12.2018 16:11