Sequences from confirmation amplicon


Confirmation sequences for line SALK_148352c and hit At2g02080 are shown.
Line ID SALK_148352c
Locus At2g02080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:520658
Primers used for PCR 29rb - LBb3, show primer details
   
Sequence Name 86-K116055-SALK_148352C-29rb
GenBank Accession LN486298 [GenBank]
Sequencing primer gene specific primer, 29rb: 5'-GATTCAACAACCTAACTCCTCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K116055-SALK_148352C-29rb
CACCCGGAACCCAGCAAGTATCTCTTACATATAAATCTCTATATATTTATCACNCTTTCT
ATAAGAATCACTATATATAAGCTTTAAGATAATACCAAGAAGAAAAAGAACCCTAATCAT
TTGTTCTATTTTGTTTTGTATATCTAGCTAGTATCAAGATTTCATATATATTGACAGGCC
AGTATATCTTATATGTTTATACGTGTATATGTCATTTGATTTATGTTTACATGTGTGTGT
ATCATTTTTTTCGATATAGTCTACATAGATTGACATTTTGGGTCTAATTGGTGACTTAAT
TTAAATGATTAAATTCTTTAATTATATGAAACAGATCCTGATGCGGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATGGGGCAAAATAAAAAAAA
AAA
   
Sequence Name 59-K116054-SALK_148352C-R210
GenBank Accession LN485722 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K116054-SALK_148352C-R210
AGATCTGTTTCAATAATTAAAGAATTTAATCATTTAAATTAAGTCACCAATTAGACCCAA
AATGTCAATCTATGTAGACTATATCGAAAAAAATGATACACACACATGTAAACATAAATC
AAATGACATATACACGTATAAACATATAAGATATACTGGCCTGTCAATATATATGAAATC
TTGATACTAGCTAGATATACAAAACAAAATAGAACAAATGATTAGGGTTCTTTTTCTTCT
TGGTATTATCTTAAAGCTTATATATAGTGATTCTTATAGAAAGGTGATAAATATATAGAG
ATTTATATGTAAGAGATACTTGCTTGGGTTTCCGGGTTGGTTTCTTCGTTTCTTGGGTGG
TGGAGCTGAGGAGTTAGGTTGTTGAATCA

Last Updated on 06.12.2018 16:11