Sequences from confirmation amplicon


Confirmation sequences for line SALK_150143 and hit At4g14510 are shown.
Line ID SALK_150143
Locus At4g14510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8337828
Primers used for PCR 67xk - LBb3, show primer details
   
Sequence Name 76-K119133-SALK_150143C-67xk
GenBank Accession LN486164 [GenBank]
Sequencing primer gene specific primer, 67xk: 5'-ATTGAGTTAAGAGATTGCTCCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K119133-SALK_150143C-67xk
AAAGCTGCTGAAAGCTGAACTGGGCTTTAGCTAGGTGGAGGAATCTCTGAAGCCAGCAGA
GCAGAGAACAGACCTTGAGGGCATAACAGAAGAAGAGAGATTTATGTTCCAAAAGCTCGG
ATTGAAGATGAAAGCTTTCTTACTTCTTGGTAAACCATTTTATTATCAATATGTGGGCTG
ATGTAGTTAAGTATGTTTGACCTGTGTTCTCTGGGATATTGACTTGTCTGAGAAACTTGA
ATTGATTGCTCCAGGTCGAAGAGGGGTGTTTGATGGGACTGTAGAGAACATGCACTTGCA
CTGGAAATATCGGGAGCTGATCAAGATTCTTGTAAAGGCTAAAACTTTAGAGGGTGCACA
GAAAGTGGCAATGGCCCTTGAAGCCGAGAGTGGAGGTATCCTGGTTTCTGTTGACAAAAT
TTCCAAAGGCTATGCCGTAATCGTGTATAGAGGAAAAGATTATAAACGTCCTACCACACT
GAGGCCTAAAAACCTCTTGACAAAGAGGAAAGCTTTGGCTCGTTCTCTTGAGCTTCAAAA
ACGCGAGGTTCGTGATAAAAATATATGCAACAACGTGTGTTGATCTCAAAATCTGCAAAC
TTACCATTGATTTCTACTTGTTATGACCAACACCTGCGTCTAATCAAGTTTAAACCTCAC
CGGCTAACACTGATTTTGCTTTCTTATTTGGATACTTTGCATTCAACAGGCTCTTATTAA
ACACATTGAGGCGATACAGACGAGATCAGAGCAGCTGAGAGCTGAAATTGTATGGTTCCA
TCTATCTGTAGCATTCACTAATCAAACAAAGAACGCATTTTCTTGTTCTGCAATGTTTGC
TAACCCGAGTCCC
   
Sequence Name 62-K119132-SALK_150143C-R210
GenBank Accession LN485807 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K119132-SALK_150143C-R210
GTCATTTGTTTAGTATGAATCTACTANCTTCTAAAGCTTAAATTCTTTGCATGTGATGCA
CTTCGTATCTCTCATTGATTGCTCAACCAAGAACAAGATGTCAAAACTTATCTACTGCAT
CAATTCACTGCAGTGTGCACACAAGCCGTTCTTTATTACCACTTATGGTTGTACATCAAG
TTCCTCATTCTGCAGTTCTACAAAGGTTGTTTCTGGTGTTGTAGAGGATGCAGAATTATC
GCCAAAATCTGTGTCTGATTCATCTGAACCAAATTCAACATCTGTTTCAGATAGTGAACC
GTTGGCCTGAATCCCACCTTCTTCATCATCTTCTCCCTCGTCTTCGTATGTATCTAAATA
CACGTCATCTTCCTCTCCCTGCAACAGCCAAAAGAGAACAAGGAATATGAAAGACATCAA
ACACAACATTCATGATAGTGACAATTCAAGGAAATAAAGAGAGACGTATAAGGCAAAAAC
ATCATACATCTGTTTCTTCTGTTTCCTCGTCAGAAGAATAAGCCATGTCAAGCTTGTCGT
ATAATGTTTCATCTCCCTTGTCTTTTACAAGTTCCACTTGTTCCTGAAAGAATGAATTCA
GGGACTCGGGTTAGCAAACATTGCAGAACAAGAAAATGCGTTCTTTGTTTGATTAGTGAA
TGCTACAGATAGATGGAACCATACAATTTCAGCTCTCAGCTGCTCTGATCTCGTCTGTAT
CGCCTCAATGTGTTTAATAAGAGCCTGTTGAATGCAAAGTATCCAAATAAGAAAGCAAAA
TCAGTGTTAGCCGGTGAGGTTTAAACTGATTAGACGCAGGTGTTGGTCATAACAAGTAGA
AATCAATGGTAAGTTTGCAGATTTTG

Last Updated on 06.12.2018 16:11