Sequences from confirmation amplicon


Confirmation sequences for line SALK_150636c and hit At3g58170 are shown.
Line ID SALK_150636c
Locus At3g58170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21543776
Primers used for PCR 30rb - LBb3, show primer details
   
Sequence Name 88-K116055-SALK_150636C-30rb
GenBank Accession LN486320 [GenBank]
Sequencing primer gene specific primer, 30rb: 5'-CAAGTTAACCATCGATGAAACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K116055-SALK_150636C-30rb
TCAGCTTCGTCGGCGTTTGAAAATCTGGACGACGGTGTAACAGCGCCGTCGACTAGGGTT
TTTGTATACAAAAGAGAAGAGAATCTGATTCGTGAAAAAGAGAAAAAAAACGCGGGACCT
ATTCCGGAAATAACGAAATCTATATTTGGTCCAAAACAATTTATTGATTTTAAATTTGAC
CCCATAGTTTCTATTATCCATTACTTGCGTATACACGTGTTGTTGAGTGTTGATTCGCTT
AAGAACGATAAACACAGAGCTTTCTGTTCTTTCGACATATCAACCGTTTCCTTCCTTAAT
TTGCGTTCGTTGTGAAACCATAGATCCAGGTGAATTCTGGAAAACATCTTGAAGCGAAAT
CGAGACATCGTTGGAGGATCAAAACGTTAATTCGATTGATCGATGAATCCTAGAAGGTTA
GCTCTCCTTGAATGTACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAT
CGGGCAAAAAT
   
Sequence Name 61-K116054-SALK_150636C-R210
GenBank Accession LN485773 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K116054-SALK_150636C-R210
CGATAATCGAATTAACGTTTTGATCCTCCACGATGTCTCGATTTCGCTTCAAGATGTTTT
CCAGAATTCACCTGGATCTATGGTTTCACAACGAACGCAAATTAAGGAAGGAAACGGTTG
ATATGTCGAAAGAACAGAAAGCTCTGTGTTTATCGTTCTTAAGCGAATCAACACTCAACA
ACACGTGTATACGCAAGTAATGGATAATAGAAACTATGGGGTCAAATTTAAAATCAATAA
ATTGTTTTGGACCAAATATAGATTTCGTTATTTCCGGAATAGGTCCCGCGTTTTTTTTCT
CTTTTTCACGAATCAGATTCTCTTCTCTTTTGTATACAAAAACCCTAGTCGACGGCGCTG
TTACACCGTCGTCCAGATTTTTCAAACGCCGACGAAGCTGATTTAGTGAATATGGAATCT
AATGGATCAGTTTCATCGATGGTTAACTTG

Last Updated on 06.12.2018 16:11