Sequences from confirmation amplicon


Confirmation sequences for line SALK_151293c and hit At4g07390 are shown.
Line ID SALK_151293c
Locus At4g07390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:4196810
Primers used for PCR 51wj - LBb3, show primer details
   
Sequence Name 26-K118627-SALK_151293C-51wj
GenBank Accession LN485047 [GenBank]
Sequencing primer gene specific primer, 51wj: 5'-TACCTTTGCAGCTTTAATCTTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118627-SALK_151293C-51wj
CACAACTTGGATAGACCACTTCTGTATCCTTTTTGGTTATAGGAATTTCTCAACTTTGAT
TTGAGACTCTCTGTTTTAGTTTTTTCTTAATGTTACGCCGATATAGATATTGCGCTGTGG
CGCCAACTGTGCTTGCTGGACAGATTAATCCGACGCTTTTCGAAGCTCTTTATGTGAGGA
AGAAGTTCTTGTTTTATTTACTTTAGGCTCTGTGTGTGTGTGTGTGTGTGTGTGTGGATA
GATTGTGATAGTGTATATTTGTGTTGTAGGCTTCACAACATGCGATATTTCTCTTTGCAA
GACTCCCTCAGATATGGAAGAACTTTAAAGTGAGTCTATACTACAATACTCATTTTCACT
ATTTTTGTGATTGATGTCTCTTCTTGTTGAACTTGGTGTTTTGAGCTTAAGTCACGTGTT
AATGTTCAGTCATGCCTTTATGTTCCTGTGATCAATCTATAGTTAAACTTTTATAGTATA
GTTTACCTTACAATCTTTTGCTGGATACAAACACTGTTATTATTGTTCATGATTCATAGA
TACTCGTTTTAGCCAACACTGTTATTATTGTTCAGGATATATTGTGGTGTAAACAAATTG
ACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCT
TTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTG
CAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
   
Sequence Name 15-K118626-SALK_151293C-R210
GenBank Accession LN484709 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118626-SALK_151293C-R210
CATTTGTTTACCCACAATATATCCTGAACATAATAACAGTGTTGGCTAAAACGAGTATCT
ATGAATCATGAACAATAATAACAGTGTTTGTATCCAGCAAAAGATTGTAAGGTAAACTAT
ACTATAAAAGTTTAACTATAGATTGATCACAGGAACATAAAGGCATGACTGAACATTAAC
ACGTGACTTAAGCTCAAAACACCAAGTTCAACAAGAAGAGACATCAATCACAAAAATAGT
GAAAATGAGTATTGTAGTATAGACTCACTTTAAAGTTCTTCCATATCTGAGGGAGTCTTG
CAAAGAGAAATATCGCATGTTGTGAAGCCTACAACACAAATATACACTATCACAATCTAT
CCACACACACACACACACACACACACAGAGCCTAAAGTAAATAAAACAAGAACTTCTTCC
TCACATAAAGAGCTTCAAAAAGCGTCAGATTAATCTGTCCAGCACGCACAGTTGGCGCCA
CAGCGCAATATCTATATGGGCGTAACATTAAGAAAAAACTAACACAGAGAGTCTCAAATC
AAAGTTGAGAAATTCCTATCACCAAAAAGGATACAGAAGTGGTCTAATCCAAGTTGTGAC
AGGTACTGGTTGTGAATAATAATAGATACGAGCCACCAAGATTAACGCTGCC

Last Updated on 06.12.2018 16:11